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S100A10 Antibody (344015) [Unconjugated]

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Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated ...read more
S100A10 was detected in immersion fixed TK‑1 mouse T cell lymphoma cell line using Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) at 5 µg/mL for 3 hours at room temperature. Cells were ...read more
Raw264 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
344015
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2377U-100UG
Alexa Fluor 405
FAB2377V-100UG
Alexa Fluor 488
FAB2377G-100UG
Alexa Fluor 532
FAB2377X
Alexa Fluor 594
FAB2377T-100UG
Alexa Fluor 647
FAB2377R-100UG
Alexa Fluor 700
FAB2377N-100UG
Alexa Fluor 750
FAB2377S-100UG
Allophycocyanin
FAB2377A
Allophycocyanin/Cy7
FAB2377APCCY7
Biotin
FAB2377B
CoraFluor 1
FAB2377CL1
DyLight 350
FAB2377D
DyLight 405
FAB2377E
DyLight 488
FAB2377K
DyLight 550
FAB2377L
DyLight 594
FAB2377M
DyLight 650
FAB2377W
DyLight 680
FAB2377Y
DyLight 755
FAB2377Z
FITC
FAB2377F
HRP
FAB2377H
Janelia Fluor 525
FAB2377JF525
Janelia Fluor 549
FAB2377I
Janelia Fluor 585
FAB2377JF585
Janelia Fluor 635
FAB2377JF635
Janelia Fluor 646
FAB2377J
Janelia Fluor 669
FAB2377JF669
PE
FAB2377P
PE/Atto594
FAB2377PEATT594
PE/Cy5.5
FAB2377PECY55
PE/Cy7
FAB2377PECY7
PerCP
FAB2377C
mFluor Violet 450 SE
FAB2377MFV450
mFluor Violet 500 SE
FAB2377MFV500
mFluor Violet 610 SE
FAB2377MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2377-SP
Carrier Free
FAB2377V-100UG
Carrier Free
FAB2377U-100UG
Carrier Free
FAB2377T-100UG
Carrier Free
FAB2377S-100UG
Carrier Free
FAB2377R-100UG
Carrier Free
FAB2377N-100UG
Carrier Free
FAB2377G-100UG
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S100A10 Antibody (344015) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse S100A10
Met1-Lys97
Accession # P08207
Specificity
Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
S100A10
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB2377 in the following applications:

  • ICC
    1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A10 Antibody (344015) [Unconjugated]

  • annexin II ligand, calpactin I, light polypeptide
  • ANX2L
  • ANX2LG
  • CAL1LGP11,42C
  • Calpactin I light chain
  • Calpactin-1 light chain
  • Cellular ligand of annexin II
  • CLP11Ca[1]
  • MGC111133
  • p10 protein
  • p10
  • P11
  • protein S100-A10
  • S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
  • S100 calcium binding protein A10
  • S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
  • S100 calcium-binding protein A10
  • S100A10

Background

S100A10, also called p11, is a 97 amino acid member of the S100 family of EF hand proteins, but does not bind calcium. It is primarily found, either within or on the surface of mast cells, as a complex of two annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is a plasminogen receptor important for plasmin production and cellular invasiveness. Intracellular S100A10 may target ligands to the endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with human S100A10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for S100A10 Antibody (MAB2377)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC, IHC.


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ICC
(1)
IHC
(1)
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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol S100A10
Uniprot