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RSK3 Antibody (3C4C8) - BSA Free

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Western Blot: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis using RSK3 mouse mAb against Hela (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
Immunocytochemistry/ Immunofluorescence: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis of A431 cells using RPS6KA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis of paraffin-embedded lung cancer tissues using RPS6KA2 mouse mAb with DAB staining.
Flow Cytometry: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis of Hela cells using RPS6KA2 mouse mAb (green) and negative control (red).
Western Blot: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis using RPS6KA2 mAb against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
Western Blot: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis using RPS6KA2 mAb against HEK293 (1) and RPS6KA2 (AA: 415-734)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: RSK3 Antibody (3C4C8) [NBP2-52555] - Analysis of paraffin-embedded cervical cancer tissues using RPS6KA2 mouse mAb with DAB staining.
ELISA: RSK3 Antibody (3C4C8) [NBP2-52555] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3C4C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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RSK3 Antibody (3C4C8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human RSK3 (AA: 415-734) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPS6KA2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
83.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-52555.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RSK3 Antibody (3C4C8) - BSA Free

  • EC 2.7.11
  • EC 2.7.11.1
  • HU-2
  • MAP kinase-activated protein kinase 1c
  • MAPK-activated protein kinase 1c
  • MAPKAP kinase 1c
  • MAPKAPK1C
  • MAPKAPK-1c
  • p90-RSK 2
  • p90RSK2
  • p90-RSK3
  • ribosomal protein S6 kinase alpha 2
  • ribosomal protein S6 kinase alpha-2
  • ribosomal protein S6 kinase, 90kD, polypeptide 2
  • ribosomal protein S6 kinase, 90kDa, polypeptide 2
  • Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2
  • RPS6KA2
  • RSK
  • RSK3
  • RSK-3
  • RSK3pp90RSK3
  • S6K-alpha
  • S6K-alpha2
  • S6K-alpha-2

Background

RSK3 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RSK3 Antibody (NBP2-52555)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPS6KA2