Novus Biologicals products are now on bio-techne.com

RPL3 Antibody

Images

 
Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: RPL3 Antibody [NBP1-81332] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear(nucleolar) positivity in neuronal cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

RPL3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHR
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RPL3 Protein (NBP1-81332PEP)
Publications
Read Publication using NBP1-81332.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19454283)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RPL3 Antibody

  • 60S ribosomal protein L3
  • HIV-1 TAR RNA-binding protein B
  • MGC104284
  • OK/SW-cl.32
  • ribosomal protein L3
  • RPL3
  • TARBP-B

Background

RPL3, also known as 60S ribosomal protein L3, is a 46 kDa 403 amino acid protein found in the cytoplasm. The primary function of RPL3 is believed to contribute to tat-mediated transactivation. Research is currently being conducted on RPL3 in relation to Treacher Collins syndrome, kidney disease, esophageal carcinoma, ovarian carcinoma, esophagitis, colon cancer, prostate cancer, carcinoma, prostatitis and malaria. The gene has been linked to the pathogenesis, innervation, translation, transport and metabolism of mRNA pathways where it interacts with RPS3, RPL4, RPL8, RPL11, and RPL12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00006124-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
H00058516-B02P
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-83192
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80104
Species: Hu
Applications: WB
NBP1-88582
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31413
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-20216
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
MAB8164
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC
NBP1-81328
Species: Hu
Applications: ICC/IF, WB
NBP2-38323
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF750
Species: Mu
Applications: AdBlk, WB
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB300-164
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, PLA, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
NBP2-25200
Species: Hu
Applications: B/N, Flow, IHC, IHC-Fr, WB
NBP1-86616
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-58917
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-25265
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NB100-2269
Species: Hu, Mu
Applications: IHC, IHC-P, IP, KD, WB
NBP1-81332
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for RPL3 Antibody (NBP1-81332)(1)

Reviews for RPL3 Antibody (NBP1-81332) (0)

There are no reviews for RPL3 Antibody (NBP1-81332). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RPL3 Antibody (NBP1-81332) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional RPL3 Products

Research Areas for RPL3 Antibody (NBP1-81332)

Find related products by research area.

Blogs on RPL3

There are no specific blogs for RPL3, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RPL3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RPL3