![Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-Paraffin-NB100-1534-img0002.jpg "Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
| Reactivity | Hu, Mu, CaSpecies Glossary |
| Applications | ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-KQPIVDTSAES corresponding to C-Terminus according to NP_689969.2. |
| Predicted Species | Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | RICTOR |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Preliminary experiments gave an approx. 38 kDa band in human lung carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192 kDa band according to NP_689969.2. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Rictor Antibody (NB100-1534)Find related products by research area.
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mTOR - a central regulator of cell metabolism The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp... Read full blog post. |
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![Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-NB100-1534-img0003.jpg "Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY license.")
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