Reactivity | Hu, Mu, Rt, Am, Bv, ZeSpecies Glossary |
Applications | WB, ELISA, Func, ICC/IF, IHC, IP |
Clone | Rho 1D4 |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Alexa Fluor 594 |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Bleached bovine rod outer segment (ROS). |
Specificity | The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold. |
Isotype | IgG Kappa |
Clonality | Monoclonal |
Host | Rabbit |
Gene | RHO |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Optimal dilution of this antibody should be experimentally determined. |
Storage | Store at 4C in the dark. |
Buffer | 50mM Sodium Borate |
Preservative | 0.05% Sodium Azide |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Rhodopsin Antibody (NBP2-81059AF594)Find related products by research area.
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Vision Infographic: Do you see how I see? Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological... Read full blog post. |
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Gene Symbol | RHO |