Rhodopsin Antibodies
We offer Rhodopsin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each Rhodopsin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Rhodopsin Antibodies can be used in a variety of model species: Amphibian, Avian, Bovine, Canine, Fish, Human, Mammal, Mouse, Porcine, Rat, Reptile, Xenopus. Use the list below to choose the Rhodopsin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Rhodopsin polyclonal antibodies and browse our Rhodopsin monoclonal antibody catalog.
Alternate Names for Rhodopsin Antibodies
anti-Rhodopsin antibody, anti-RHO antibody, anti-CSNBAD1 antibody, anti-MGC138309 antibody, anti-OPN2MGC138311 antibody, anti-opsin 2, rod pigment antibody, anti-opsin-2 antibody, anti-retinitis pigmentosa 4, autosomal dominant antibody, anti-RP4 antibody
10 Results for "rhodopsin" in Primary Antibodies
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0005.jpg "Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.")
![Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-NBP2-59690-img0006.jpg)
![Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug.. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Western-Blot-NBP2-59690-img0004.jpg)
![Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-4D2-Immunohistochemistry-Frozen-NBP2-59690-img0002.jpg)
![Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunohistochemistry-NBP2-25160-img0004.jpg "Immunohistochemistry: Rhodopsin Antibody (B630) [NBP2-25160] - Rhodopsin expression and the prevalence of rod photoreceptors in the canine retina. (A) Fluorescence micrographs showing rhodopsin expression (red) in the ABCA4+/+ (left), ABCA4+/- (middle), and ABCA4-/- (right) rod outer segments. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.")
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication. 50 ug of protein samples were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with the following primary antibodies: ABCA4 (NBP1-30032, 1:1000) and Rhodopsin (NBP2-25160, 1:5000), followed by Anti-Mouse IgG horseradish peroxidase-conjugated secondary antibody (R&D Systems, HAF007, 1:5000. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (B630) [NBP2-25160] - High magnification confocal image of pig retinal section stained with NBP2-25160 (Green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue).](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Immunocytochemistry-Immunofluorescence-NBP2-25160-img0002.jpg)
![Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important to avoid boiling samples containing this protein in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the Rhodopsin protein and appearance of more of this high molecular weight material.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-B630-Western-Blot-NBP2-25160-img0003.jpg)
![Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-A531-Western-Blot-NBP2-25159-img0004.jpg "Western Blot: Rhodopsin Antibody (A531) [NBP2-25159] - Blot of bovine retinal extracts. The antibody stains a band corresponing to retinal Rhodopsin at about 35kDa. Bands about 70 kDa and 140 kDa are aggregated forms of Rhodopsin. Note, due to the highly hydrophobic nature of Rhodopsin, it is important not to boil a sample containing it in SDS-PAGE sample buffer, as this will result in more extensive aggregation of the Rhodopsin protein.")
![Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (A531) [NBP2-25159] - Pig retinal section stained with NBP2-25159 (green) and counterstained with rabbit polyclonal antibody to NF-M NB300-133 (red) and DNA (blue). Rhodopsin is most abundant in the outer segments of retina (OS), NF-M is abundant in the optic nerve fiber layer (ONFL), but seen in processes and neurons in other regions also. Other layers are pigmented epithelium (PE), outer and inner nuclear layers (ONL, INL), outer and inner plexiform layers (OPL, IPL) and ganglion cell layer (GCL).](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-A531-Immunocytochemistry-Immunofluorescence-NBP2-25159-img0003.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0007.jpg "Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Quantification of rhodopsin protein (western blot) levels in the neural retina of WT (n = 7) and hFATP1TG (n = 8) mice. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28672005/) licensed under a CC-BY license.")
![Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Immunohistochemistry-NBP1-47602-img0006.jpg)
![Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4 (NBP1-47602). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP1-47602) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-1D4-Western-Blot-NBP1-47602-img0005.jpg)
![Western Blot: Rhodopsin Antibody (RET-P1) [NB120-3267] - Analysis of IMR-5 Cells.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-RET-P1-Western-Blot-NB120-3267-img0004.jpg "Western Blot: Rhodopsin Antibody (RET-P1) [NB120-3267] - Analysis of IMR-5 Cells.")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody (RET-P1) [NB120-3267] - Human brain stained with Rhodopsin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of neurons.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-RET-P1-Immunohistochemistry-Paraffin-NB120-3267-img0005.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0004.jpg "Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Analysis of anti-RHO / Rhodopsin antibody with human eye, retina.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0002.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NLS1052] - Eye, Retina](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NLS1052-img0003.jpg)
![ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Rho-1D4-Chimeric-ELISA-NBP3-09001-img0001.jpg "ELISA: Rhodopsin Antibody (Rho 1D4) - Chimeric [NBP3-09001] - Using 1D4 (Ab00337) and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Rhodopsin antibody 1D4 (Ab00337-1.1; dark blue line) and isotype control (Ab00102-1.1, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 (Pr00565, Absolute Antibody) at a concentration of 5 ug/ml. Ab02007 antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.")
![Western Blot: Rhodopsin Antibody (Rho 1D4) [NBP2-81059] - Western Blot using anti-Rhodopsin antibody (Rho 1D4) [NBP2-81059]. Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 [NBP2-81059] at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat ( Rattus norvegicus ) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). NBP2-81059 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Rho-1D4-Western-Blot-NBP2-81059-img0001.jpg "Western Blot: Rhodopsin Antibody (Rho 1D4) [NBP2-81059] - Western Blot using anti-Rhodopsin antibody (Rho 1D4) [NBP2-81059]. Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of Rho 1D4 [NBP2-81059] at 0.0001 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat ( Rattus norvegicus ) Rhodopsin is 39.0kDa, though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al., PMID: 16492772, PMID: 10798675). NBP2-81059 successfully detected monomeric, dimeric and multimeric Rhodopsin in rat eye lysate.")
![Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Western blot analysis of extracts of various cell lines, using Rhodopsin Rabbit mAb (NBP3-16655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-5N3G9-Western-Blot-NBP3-16655-img0002.jpg "Western Blot: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Western blot analysis of extracts of various cell lines, using Rhodopsin Rabbit mAb (NBP3-16655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Rhodopsin Antibody (5N3G9) [NBP3-16655] - Immunohistochemistry of paraffin-embedded mouse retina using Rhodopsin Rabbit mAb (NBP3-16655) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-5N3G9-Immunohistochemistry-Paraffin-NBP3-16655-img0001.jpg)
![Immunohistochemistry-Paraffin: Rhodopsin Antibody [NBP2-55553] - Staining of human retina shows strong cytoplasmic positivity in rod segments.](http://images.novusbio.com/fullsize/Rhodopsin-Antibody-Immunohistochemistry-Paraffin-NBP2-55553-img0003.jpg "Immunohistochemistry-Paraffin: Rhodopsin Antibody [NBP2-55553] - Staining of human retina shows strong cytoplasmic positivity in rod segments.")