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RhoC Antibody (1B7)

Images

 
Western Blot: RhoC Antibody (1B7) [H00000389-M06] - Analysis of RHOC expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: RhoC Antibody (1B7) [H00000389-M06] - Analysis of monoclonal antibody to RHOC on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: RhoC Antibody (1B7) [H00000389-M06] - Analysis of RHOC expression in PC-12 (Cat # L012V1).
Sandwich ELISA: RhoC Antibody (1B7) [H00000389-M06] - Detection limit for recombinant GST tagged RHOC is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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RhoC Antibody (1B7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
RHOC (AAH07245, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL
Specificity
RHOC - ras homolog gene family, member C (1B7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RHOC
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publication using H00000389-M06.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RhoC Antibody (1B7)

  • ARH9ARHCRho cDNA clone 9
  • H9
  • MGC1448
  • MGC61427
  • oncogene RHO H9
  • ras homolog gene family, member C
  • RAS-related homolog 9
  • rhoC GTPase
  • RhoC
  • RHOH9
  • rho-related GTP-binding protein RhoC
  • small GTP binding protein RhoC

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoC Antibody (H00000389-M06)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RhoC Antibody (H00000389-M06) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for RhoC Antibody (H00000389-M06)

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Blogs on RhoC.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol RHOC
Entrez
Uniprot