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Rheb Antibody (344912) [Unconjugated]

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Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
Rheb was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 3 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Rheb was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line (Positive) & PC‑12 rat adrenal pheochromocytoma cell line (Negative) using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
344912
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3426-SP
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Rheb Antibody (344912) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Rheb
Met1-Met184
Accession # AAH66307
Specificity
Detects human, mouse, and rat Rheb.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RHEB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB3426 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rheb Antibody (344912) [Unconjugated]

  • GTP-binding protein Rheb
  • MGC111559
  • Ras homolog enriched in brainRHEB2Ras homolog enriched in brain 2
  • Rheb
  • Rheb2

Background

Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rheb Antibody (MAB3426)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(1)
Western Blot
(2)
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(1)
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Product General Protocols

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Bioinformatics

Gene Symbol RHEB
Uniprot