![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Goat anti-RENT1 antibody NB100-370 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-370-img0047.jpg "Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Goat anti-RENT1 antibody NB100-370 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1075 and the C-terminus (residue 1118) of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | UPF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | By Western blot, this antibody recognizes a band at ~145 kDa, representing human RENT1. *The investigator should determine the optimal working dilution for a specific application. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-370 | Applications | Species |
|---|---|---|
| Luke B, Azzalin CM, Hug N et al. Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decay. Nucleic Acids Res 2007-01-01 [PMID: 17984081] |
Secondary Antibodies |
Isotype Controls |
Research Areas for RENT1/UPF1/hUPF1 Antibody (NB100-370)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UPF1 |
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![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-370-img0043.jpg "Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.")
![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (lot For blotting immunoprecipitated RENT1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-370-img0045.jpg "Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (lot For blotting immunoprecipitated RENT1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Western-Blot-NB100-370-img0046.jpg "Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Goat anti-RENT1 antibody NB100-370 (NB100-370 lot 4) used for IP at 6 ug per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-370-3). For blotting immunoprecipitated RENT1, NB100-370 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/RENT1-UPF1-hUPF1-Antibody-Immunoprecipitation-NB100-370-img0048.jpg "Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Goat anti-RENT1 antibody NB100-370 (NB100-370 lot 4) used for IP at 6 ug per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-370-3). For blotting immunoprecipitated RENT1, NB100-370 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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