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Renin R Antibody

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Genetic Strategies: Western Blot: Renin R Antibody [NBP1-90820] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunohistochemistry-Paraffin: Renin R Antibody [NBP1-90820] - Staining of human pancreas shows moderate cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: Renin R Antibody [NBP1-90820] - Staining of human cerebellum shows moderate cytoplasmic/ membranous positivity in cells in granular layer
Immunohistochemistry-Paraffin: Renin R Antibody [NBP1-90820] - Staining of human cerebral cortex shows moderate cytoplasmic/ membranous positivity in neurons and moderately stained neuropil.
Immunohistochemistry-Paraffin: Renin R Antibody [NBP1-90820] - Staining of human placenta shows moderate cytoplasmic/ membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Renin R Antibody [NBP1-90820] - Staining of human renal cancer shows moderate membranous positivity in tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Renin R Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NSLSRNNEVDLLFLSELQVLHDISSLLSRHKHLAKDHSPDLYSLELAGLDEIGKRYGEDSEQFRDASKILVDALQKFADDMYSLYGGNAVVELVTVKSFDTSLIRKTRTILEAKQAKNPASPYNLAYKYNFEYSVVFN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6AP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. WB, ICC/IF reported in scientific literature (PMID: 25143455).
Control Peptide
Renin R Protein (NBP1-90820PEP)
Publications
Read Publications using
NBP1-90820 in the following applications:

  • 2 publications
  • WB
    4 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23469216). Rat reactivity reported in scientific literature (PMID: 25250989), Mouse reactivity reported in scientific literature (PMID:25046440).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Renin R Antibody

  • APT6M8-9
  • ATP6AP2
  • ATP6IP2
  • ATP6M8-9
  • ATP6M8-9MRXE
  • ATPase H(+)-transporting lysosomal accessory protein 2
  • ATPase H(+)-transporting lysosomal-interacting protein 2
  • ATPase, H+ transporting, lysosomal accessory protein 2
  • ATPase, H+ transporting, lysosomal interacting protein 2
  • CAPER
  • ELDF10
  • Embryonic liver differentiation factor 10
  • ER-localized type I transmembrane adaptor
  • H+ transporting, lysosomal (vacuolar proton pump) membrane sectorassociated protein M8-9
  • HT028
  • M8-9
  • MGC99577
  • MRXE
  • MSTP009
  • N14F
  • Renin R
  • renin receptor
  • ReninR
  • vacuolar proton ATP synthase membrane sector associated protein M8-9
  • V-ATPase M8.9 subunit
  • XMRE

Background

Renin receptor encodes a protein which is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Renin R Antibody (NBP1-90820)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-90820 Applications Species
Senturk M, Lin G, et al. Ubiquilins regulate autophagic flux through mTOR signalling and lysosomal acidification. Nat Cell Biol 2019-03-01 [PMID: 30804504] (WB, Human) WB Human
Gonzalez AA, Womack JP, Liu L et al. Angiotensin II increases the expression of (pro)renin receptor during low salt conditions in rat renal inner medullary collecting ducts. Am J Med Sci 2014-11-01 [PMID: 25250989] (ICC/IF, WB, Rat) ICC/IF, WB Rat
Gonzalez AA, Green T, Luffman C et al. Renal medullary cyclooxygenase-2 and (pro)renin receptor expression during angiotensin II-dependent hypertension. Am J Physiol Renal Physiol 2014-10-15 [PMID: 25143455] (ICC/IF, WB, Rat) ICC/IF, WB Rat
Alva Rosendahl, Gianina Niemann, Sascha Lange et al. Increased expression of (pro)renin receptor does not cause hypertension or cardiac and renal fibrosis in mice. Laboratory Investigation 2014-08-01 [PMID: 25046440] (WB, Mouse) WB Mouse
KIRSCH S, SCHREZENMEIER E, KLARE S et al. The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein. Int J Mol Med 2014-04-01 [PMID: 24424509]
Zaade D, Schmitz J, Benke E et al. Distinct Signal Transduction Pathways Downstream of the (P)RR Revealed by Microarray and Chromatin Immunoprecipitation-Chromatin Immunoprecipitation Analyses. PLoS One 2013-01-01 [PMID: 23469216]
Gonzalez AA, Lara LS, Luffman C et al. Soluble form of the (pro)renin receptor is augmented in the collecting duct and urine of chronic angiotensin II-dependent hypertensive rats. Hypertension 2011-04-01 [PMID: 21321306]
Prieto MC, Williams DE, Liu L et al. Enhancement of renin and prorenin receptor in collecting duct of Cyp1a1-Ren2 rats may contribute to development and progression of malignant hypertension. Am J Physiol Renal Physiol 2011-02-01 [PMID: 21068087]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP6AP2