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Regucalcin Antibody (4B9)

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Western Blot: Regucalcin Antibody (4B9) [H00009104-M04] - Analysis of RGN expression in transfected 293T cell line by RGN monoclonal antibody (M04), clone 4B9. Lane 1: RGN transfected lysatE (33.3 KDa). Lane 2: Non-transfected lysate.
Western Blot: Regucalcin Antibody (4B9) [H00009104-M04] - Analysis of RGN expression in transfected 293T cell line by RGN monoclonal antibody (M04), clone 4B9. Lane 1: RGN transfected lysatE (33.3 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Regucalcin Antibody (4B9) [H00009104-M04] - Analysis of monoclonal antibody to RGN on HeLa cell. Antibody concentration 15 ug/ml
ELISA: Regucalcin Antibody (4B9) [H00009104-M04] - Detection limit for recombinant GST tagged RGN is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Regucalcin Antibody (4B9) Summary

Immunogen
RGN (NP_004674.1, 200 a.a. ~ 299 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQIPDGMCIDAEGKLWVACYNGGRVIRLDPVTGKRLQTVKLPVDKTTSCCFGGKNYSEMYVTCARDGMDPEGLLRQPEAGGIFKITGLGVKGIAPYSYAG
Specificity
RGN - regucalcin (senescence marker protein-30) (4B9)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RGN
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Regucalcin Antibody (4B9)

  • EC 3.1.1.17
  • gluconolactonase
  • GNL
  • RCsenescence marker protein-30
  • regucalcin (senescence marker protein-30)
  • Senescence marker protein 30
  • SMP-30
  • SMP30regucalcin

Background

The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RGN
Entrez
Uniprot