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Reg3A Antibody (512124)

 (4 Publications)
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Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Reg3A Monoclonal Antibody (Catalog # MAB5965) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Reg3A Monoclonal Antibody (Catalog # MAB5965) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Reg3A was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Reg3A Mono-clonal Antibody (Catalog # MAB5965) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
512124
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5965-SP
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Reg3A Antibody (512124) Summary

Immunogen
E. coli-derived recombinant human Reg3A
Glu27-Asp175
Accession # Q06141
Specificity
Detects human Reg3A in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
REG3A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB5965 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg3A Antibody (512124)

  • hepatocarcinoma-intestine-pancreas
  • HIPpancreatitis-associated protein
  • Human proislet peptide
  • Lithostathine 3
  • pancreatic beta cell growth factor
  • Pancreatitis-associated protein 1
  • PAP homologous protein
  • PAP1FLJ18565
  • PAP2
  • PAPPAP-H
  • PBCGF
  • proliferation-inducing protein 34
  • proliferation-inducing protein 42
  • Reg III-alpha
  • REG3
  • Reg3A
  • REG-3-alpha
  • regenerating islet-derived 3 alpha
  • regenerating islet-derived protein 3-alpha
  • Regenerating islet-derived protein III-alpha
  • REG-III

Background

Reg3A, also named Pancreatitis-Associated Protein (PAP) and peptide 23, belongs to the Regenerating gene family (Reg), within the superfamily of C-type lectins. It has been classified as a member of the type III subclass of the Reg family. Reg3A has anti-apoptotic effects on pancreatic acinar cells and is a novel motor and sensory neuron survival factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Reg3A Antibody (MAB5965)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


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(1)
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Bioinformatics

Gene Symbol REG3A
Uniprot