Recombinant Rat Thrombopoietin Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c‑mpl. Defects in the Tpo‑Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). Mature rat Tpo shares 68% and 81% aa sequence homology with human and mouse Tpo, respectively (4). It is an 80‑85 kDa protein that consists of an N‑terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N‑linked and O‑linked glycosylation sites. Tpo promotes the differentiation, proliferation, and maturation of megakaryocytes (MK) and their progenitors (5‑7). Several other cytokines can also promote these functions but only in cooperation with Tpo (8, 9). Notably, IL‑3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10‑13). These actions, in combination with direct effects on cardiomyocytes, can aid in the recovery of heart function following myocardial infarction (14). Tpo is cleaved by platelet‑derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (15). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (6). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia‑sensitized neurons and inhibits neuronal differentiation by blocking NGF‑induced signaling (16, 17).

Array 7864-TP/CF

• Thrombopoietin/THPO Antibodies
• Thrombopoietin/THPO ELISA Kit
• Thrombopoietin/THPO Lysate
• Thrombopoietin/THPO Protein
• Thrombopoietin/THPO Proteins and Enzymes
• Thrombopoietin/THPO ELISAs