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Recombinant Porcine IL-18/IL-1F4 Protein, CF

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Catalog# & Formulation Size Price
Standard Formulation
588-PL-025
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Recombinant Porcine IL-18/IL-1F4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha . The ED50 for this effect is 10-40 ng/mL in the presence of 20 ng/mL rhTNF-alpha .
Source
E. coli-derived porcine IL-18/IL-1F4 protein
Tyr36-Asn192
Accession #
N-terminal Sequence
Tyr36
Protein/Peptide Type
Recombinant Proteins
Gene
IL18
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
588-PL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and DTT with Trehalose.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Porcine IL-18/IL-1F4 Protein, CF

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature porcine IL-18 shares 88% amino acid sequence identity with canine and feline IL-18 and 61%-76% with human, mouse, rat,  and rhesus IL-18 (15).
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Muneta, Y. et al. (2000) Cytokine 12:566.

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Publications for IL-18/IL-1F4 (588-PL/CF)(6)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
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Porcine
(6)
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Showing Publications 1 - 6 of 6.
Publications using 588-PL/CF Applications Species
B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation Microorganisms, 2021-08-28;9(9):. 2021-08-28 [PMID: 34576723] (Bioassay, Porcine) Bioassay Porcine
B Holzer, P Rijal, A McNee, B Paudyal, V Martini, B Clark, T Manjegowda, FJ Salguero, E Bessell, JC Schwartz, K Moffat, M Pedrera, SP Graham, A Noble, M Bonnet-Di, RM La Ragione, W Mwangi, P Beverley, JW McCauley, RS Daniels, JA Hammond, AR Townsend, E Tchilian Protective porcine influenza virus-specific monoclonal antibodies recognize similar haemagglutinin epitopes as humans PloS Pathogens, 2021-03-04;17(3):e1009330. 2021-03-04 [PMID: 33662023] (Bioassay, Porcine) Bioassay Porcine
Mair K, Mullebner A, Essler S, Duvigneau J, Storset A, Saalmuller A, Gerner W Porcine CD8alphadim/-NKp46high NK cells are in a highly activated state. Vet Res, 2013-03-01;44(0):13. 2013-03-01 [PMID: 23452562] (Bioassay, Porcine) Bioassay Porcine
Mair KH, Essler SE, Patzl M, Storset AK, Saalmuller A, Gerner W NKp46 expression discriminates porcine NK cells with different functional properties. Eur. J. Immunol., 2012-05-01;42(5):1261-71. 2012-05-01 [PMID: 22539298] (Bioassay, Porcine) Bioassay Porcine
Cordes H, Riber U, Jensen TK, Jungersen G Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis. Vet. Res., 2012-02-08;43(1):9. 2012-02-08 [PMID: 22316065] (Bioassay, Porcine) Bioassay Porcine
Toka FN, Nfon CK, Dawson H, Mark Estes D, Golde WT Activation of Porcine Natural Killer (NK) Cells and Lysis of Foot-and-Mouth Disease Virus (FMDV) Infected Cells. J. Interferon Cytokine Res., 2009-03-01;0(0):. 2009-03-01 [PMID: 19196070] (Bioassay, Porcine) Bioassay Porcine

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Bioinformatics

Gene Symbol IL18
Uniprot