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Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein, CF

 (10 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-30 ng/mL in the presence of 5 ng/mL recombinant mouse VEGF.
Source
Mouse myeloma cell line, NS0-derived mouse VEGFR2/KDR/Flk-1 protein
Mouse VEGFR2
(Ala20-Glu762)
Accession # P35918		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Kdr
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
110 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
166-191 kDa, reducing conditions
Publications
Read Publications using
443-KD/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein, CF

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 Fc Chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.

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Recombinant Mouse
VEGFR2/Flk-1 Fc Chime ...
443-KD/CF
Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein, CF
Species: Mu
Applications: Bioactivity

Publications for VEGFR2/KDR/Flk-1 (443-KD/CF)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Llama.

We have publications tested in 6 applications: Binding Assay, Bioassay, Flow Cytometry, In Vivo, Surface Plasmon Resonance, Western Blot.


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Binding Assay
(1)
Bioassay
(4)
Flow Cytometry
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Llama
(1)
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Showing Publications 1 - 10 of 10.
Publications using 443-KD/CF Applications Species
V Mashayekhi, KT Xenaki, PMP van Bergen, S Oliveira Dual Targeting of Endothelial and Cancer Cells Potentiates In Vitro Nanobody-Targeted Photodynamic Therapy Cancers, 2020-09-23;12(10):. 2020-09-23 [PMID: 32977602] (Bioassay, Llama) Bioassay Llama
K Fujiwara, A Tsunei, H Kusabuka, E Ogaki, M Tachibana, N Okada Hinge and Transmembrane Domains of Chimeric Antigen Receptor Regulate Receptor Expression and Signaling Threshold Cells, 2020-05-09;9(5):. 2020-05-09 [PMID: 32397414] (Flow Cytometry, Mouse) Flow Cytometry Mouse
T Ikeuchi, S de Vega, P Forcinito, AD Doyle, J Amaral, IR Rodriguez, E Arikawa-Hi, Y Yamada Extracellular Protein Fibulin-7 and Its C-Terminal Fragment Have In Vivo Antiangiogenic Activity Sci Rep, 2018-12-05;8(1):17654. 2018-12-05 [PMID: 30518776] (Bioassay, Human) Bioassay Human
Naoki Okada Immunological quality and performance of tumor vessel-targeting CAR-T cells prepared by mRNA-EP for clinical research Mol Ther Oncolytics, 2016-11-16;3(0):16024. 2016-11-16 [PMID: 27909701] (Bioassay, Mouse) Bioassay Mouse
Fleetwood F, Klint S, Hanze M, Gunneriusson E, Frejd F, Stahl S, Lofblom J Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity. Sci Rep, 2014-12-17;4(0):7518. 2014-12-17 [PMID: 25515662] (Bioassay) Bioassay
Wuest TR, Carr DJ VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis. J. Exp. Med., 2009-12-21;207(1):101-15, S1-2. 2009-12-21 [PMID: 20026662] (In Vivo, Mouse) In Vivo Mouse
Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1. J. Biol. Chem., 2008-10-15;283(49):34250-9. 2008-10-15 [PMID: 18922791] (Binding Assay, Mouse) Binding Assay Mouse
Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Chem. Biol., 2006-05-01;13(5):549-56. 2006-05-01 [PMID: 16720276] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Schmitz V, Vilanueva H, Raskopf E, Hilbert T, Barajas M, Dzienisowicz C, Gorschluter M, Strehl J, Rabe C, Sauerbruch T, Prieto J, Caselmann WH, Qian C Increased VEGF levels induced by anti-VEGF treatment are independent of tumor burden in colorectal carcinomas in mice. Gene Ther., 2006-04-13;13(16):1198-205. 2006-04-13 [PMID: 16617302] (Western Blot) Western Blot
Raskopf E, Dzienisowicz C, Hilbert T, Rabe C, Leifeld L, Wernert N, Sauerbruch T, Prieto J, Qian C, Caselmann WH, Schmitz V Effective angiostatic treatment in a murine metastatic and orthotopic hepatoma model. Hepatology, 2005-06-01;41(6):1233-40. 2005-06-01 [PMID: 15915456] (Western Blot) Western Blot

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Bioinformatics

Gene Symbol Kdr
Uniprot