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Recombinant Human ST2/IL-33R Fc Chimera Protein, CF

 (12 Publications)
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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human ST2/IL-33R Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-33-dependent proliferation in D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.03-0.12 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human ST2/IL-33 R protein
Human ST2
(Lys19-Phe328)
Accession # BAA02233		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Lys19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL1RL1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
62.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100 kDa, reducing conditions
Publications
Read Publications using
523-ST in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ST2/IL-33R Fc Chimera Protein, CF

  • DER4
  • DER4ST2growth stimulation-expressed
  • Fit-1
  • IL-1 R4
  • IL1R4
  • IL-1R4
  • IL-1RL1
  • IL-33 R
  • IL33R
  • IL-33R
  • interleukin 1 receptor-like 1
  • interleukin 1 receptor-related protein
  • interleukin-1 receptor-like 1
  • Ly84
  • MGC32623
  • Protein ST2
  • ST2
  • ST2L
  • ST2V
  • T1
  • T1homolog of mouse growth stimulation-expressed

Background

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5-8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12-14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33’s ability to signal through transmembrane ST2 (10, 13‑15).

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
  4. Li, H. et al. (2000) Genomics 67:284.
  5. Schmitz, J. et al. (2005) Immunity 23:479.
  6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
  10. Palmer, G. et al. (2008) Cytokine 42:358.
  11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

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Publications for ST2/IL-33R (523-ST)(12)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 5 applications: AlphaLISA, Bioassay, Cell Culture, Flow Cytometry, Immunoprecipitation.


Filter By Application
AlphaLISA
(1)
Bioassay
(9)
Cell Culture
(1)
Flow Cytometry
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(12)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 523-ST Applications Species
K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness Nutrients, 2021-04-28;13(5):. 2021-04-28 [PMID: 33924911] (Bioassay, Human) Bioassay Human
L Matilla, V Arrieta, E Jover, A Garcia-Peñ, E Martinez-M, R Sadaba, V Alvarez, A Navarro, A Fernandez-, A Gainza, E Santamaria, J Fernandez-, P Rossignol, F Zannad, N Lopez-Andr Soluble St2 Induces Cardiac Fibroblast Activation and Collagen Synthesis via Neuropilin-1 Cells, 2020-07-10;9(7):. 2020-07-10 [PMID: 32664340] (Cell Culture, Human) Cell Culture Human
Z Sun, B Chang, A Huang, S Hao, M Gao, Y Sun, M Shi, L Jin, W Zhang, J Zhao, G Teng, L Han, H Tian, Q Liang, JY Zhang, Z Zou Plasma levels of soluble ST2, but not IL-33, correlate with the severity of alcoholic liver disease J. Cell. Mol. Med., 2018-11-27;0(0):. 2018-11-27 [PMID: 30478965] (Bioassay, Human) Bioassay Human
AM Ramadan, E Daguindau, JC Rech, K Chinnaswam, J Zhang, GL Hura, B Griesenaue, Z Bolten, A Robida, M Larsen, JA Stuckey, CY Yang, S Paczesny From proteomics to discovery of first-in-class ST2 inhibitors active in vivo JCI Insight, 2018-07-26;3(14):. 2018-07-26 [PMID: 30046004] (AlphaLISA, Human) AlphaLISA Human
K Matsumoto, H Kouzaki, H Kikuoka, T Kato, I Tojima, S Shimizu, T Shimizu Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33 Allergol Int, 2018-06-22;0(0):. 2018-06-22 [PMID: 29941231] (Bioassay, Human) Bioassay Human
Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment Nat Commun, 2016-11-24;7(0):13589. 2016-11-24 [PMID: 27882929] (Bioassay, Human) Bioassay Human
Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016-05-04;6(0):25171. 2016-05-04 [PMID: 27142573] (Bioassay, Human) Bioassay Human
Zhang Y, Zhang C, Zhuang R, Ma Y, Zhang Y, Yi J, Yang A, Jin B IL-33/ST2 correlates with severity of haemorrhagic fever with renal syndrome and regulates the inflammatory response in Hantaan virus-infected endothelial cells. PLoS Negl Trop Dis, 2015-02-06;9(2):e0003514. 2015-02-06 [PMID: 25658420] (Bioassay, Human) Bioassay Human
Seeber S, Ros F, Thorey I, Tiefenthaler G, Kaluza K, Lifke V, Fischer J, Klostermann S, Endl J, Kopetzki E, Pashine A, Siewe B, Kaluza B, Platzer J, Offner S A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood. PLoS ONE, 2014-02-04;9(2):e86184. 2014-02-04 [PMID: 24503933] (Bioassay, Human, Rabbit) Bioassay Human, Rabbit
Gillibert-Duplantier J, Duthey B, Sisirak V, Salaun D, Gargi T, Tredan O, Finetti P, Bertucci F, Birnbaum D, Bendriss-Vermare N, Badache A Gene expression profiling identifies sST2 as an effector of ErbB2-driven breast carcinoma cell motility, associated with metastasis. Oncogene, 2011-11-21;31(30):3516-24. 2011-11-21 [PMID: 22105364] (Bioassay, Flow Cytometry, Human) Bioassay, Flow Cytometry Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol IL1RL1
Uniprot