Home » NT-3 » NT-3 Peptides and Proteins » Recombinant Human NT-3 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human NT-3 Protein, CF

 (68 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for NT-3 (267-N3/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
267-N3-005
Carrier Free
267-N3-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human NT-3 Protein, CF Summary

Additional Information
Earn double rewards for reviews
Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4.00-40.0 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-3 protein
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Gene
NTF3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
267-N3/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Customers Who Viewed This Item Also Viewed...

BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(57 Publications)
Species: Hu
Applications: BA
NT-4 [Unconjugated]
268-N4/CF
NT-4 [Unconjugated]
(9 Publications)
Species: Hu
Applications: BA
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(44 Publications)
Species: Rt
Applications: IHC, WB
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(217 Publications)
Species: Hu
Applications: Bind, BA
TrkC Antibody
[Unconjugated]
AF1404
Western Blot TrkC Antibody [Unconjugated]
Simple Western TrkC Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence TrkC Antibody [Unconjugated]
(1 Review)
(66 Publications)
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
TrkB Antibody
[Unconjugated]
AF1494
Immunohistochemistry TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
(12 Reviews)
(80 Publications)
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB

NGFR/TNFRSF16/p75NTR A ...
AF1157
Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
(26 Publications)
Species: Mu
Applications: IHC, WB
tropomyosin-3
Antibody (2E4)
H00007170-M02
Sandwich ELISA tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
(2 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
CNTF [Unconjugated]
257-NT
SDS-PAGE CNTF [Unconjugated]
Bioactivity CNTF [Unconjugated]
(63 Publications)
Species: Hu
Applications: BA
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(546 Publications)
Species: Hu
Applications: BA
LEDGF Antibody
(762815) [Unconjugate
MAB3468
Western Blot LEDGF Antibody (762815) [Unconjugated]
Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC, WB
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(76 Publications)
Species: Hu
Applications: ELISA
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Neutralization Siglec-7/CD328 Antibody [Unconjugated]
(1 Review)
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB

Publications for NT-3 (267-N3/CF)(68)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Primate, Primate - Callitrix jacchus (Common Marmoset), Transgenic Mouse.

We have publications tested in 6 applications: Bioassay, CAR-T, Cell Culture, Differentiation, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(57)
CAR-T
(1)
Cell Culture
(5)
Differentiation
(4)
ELISA (Standard)
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(38)
Mouse
(19)
Rat
(6)
Canine
(1)
Chicken
(1)
Primate
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 68. Show All 68 Publications.
Publications using 267-N3/CF Applications Species
EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798415] (Bioassay, Human, Mouse) Bioassay Human, Mouse
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
Y Tang, DJ Kwiatkowsk, EP Henske Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment Nature Communications, 2022-08-26;13(1):5018. 2022-08-26 [PMID: 36028490] (Bioassay, Mouse) Bioassay Mouse
S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 2022-08-25;13(1):5003. 2022-08-25 [PMID: 36008413] (Bioassay, Mouse) Bioassay Mouse
M Sawamura, K Imamura, R Hikawa, T Enami, A Nagahashi, H Yamakado, H Ichijo, T Fujisawa, H Yamashita, S Minamiyama, M Kaido, H Wada, M Urushitani, H Inoue, N Egawa, R Takahashi Cellular analysis of SOD1 protein-aggregation propensity and toxicity: a case of ALS with slow progression harboring homozygous SOD1-D92G mutation Scientific Reports, 2022-07-25;12(1):12636. 2022-07-25 [PMID: 35879519] (Bioassay, Human) Bioassay Human
MK Eltahir, M Nakamori, S Hattori, T Kimura, H Mochizuki, S Nagano Pharmacotherapy alleviates pathological changes in human direct reprogrammed neuronal cell model of myotonic dystrophy type 1 PLoS ONE, 2022-07-01;17(7):e0269683. 2022-07-01 [PMID: 35776705] (Bioassay, Human) Bioassay Human
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 2022-04-05;23(6):e54041. 2022-04-05 [PMID: 35384245] (Bioassay, Human) Bioassay Human
JM Suárez-Riv, CJ Pastor-Mal, A Romero-Gon, D Gómez-Fern, S Povea-Cabe, M Álvarez-Có, I Villalón-G, M Talaverón-, A Suárez-Car, M Munuera-Ca, JA Sánchez-Al Pterostilbene in Combination With Mitochondrial Cofactors Improve Mitochondrial Function in Cellular Models of Mitochondrial Diseases Frontiers in Pharmacology, 2022-03-18;13(0):862085. 2022-03-18 [PMID: 35370630] (Bioassay, Human) Bioassay Human
A Bruzelius, S Kidnapilla, J Drouin-Oue, T Stoker, RA Barker, D Rylander O Reprogramming Human Adult Fibroblasts into GABAergic Interneurons Cells, 2021-12-08;10(12):. 2021-12-08 [PMID: 34943958] (Bioassay, Human) Bioassay Human
D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021-12-21;37(12):110152. 2021-12-21 [PMID: 34936870] (Bioassay, Mouse) Bioassay Mouse
Show All 68 Publications.

Reviews for NT-3 (267-N3/CF) (0)

There are no reviews for NT-3 (267-N3/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for NT-3 (267-N3/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NT-3 Products

Blogs on NT-3

There are no specific blogs for NT-3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NT-3 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol NTF3
Uniprot