Home » NT-3 » NT-3 Peptides and Proteins » Recombinant Human NT-3 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human NT-3 Protein, CF

 Submit a Review

Images

 
(
Enlarge)
Recombinant Human NT-3 Protein (Catalog # 11346-N3) has a molecular weight (MW) of 28.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human NT-3 Protein (Catalog # 11346-N3) has a molecular weight (MW) of 28.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational ...read more
Recombinant Human NT‑3 (Catalog # 11346-N3) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 5.00-60.0 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
11346-N3-050
Carrier Free
11346-N3-100
Carrier Free
11346-N3-01M
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human NT-3 Protein, CF Summary

Additional Information
(HEK293 expressed), Analyzed by SEC-MALS. 11563-N3, a new product from mammalian cell line is available
Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 5.00-60.0 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human NT-3 protein
Tyr139-Thr257
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12-15 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Customers Who Viewed This Item Also Viewed...

BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
NT-4 [Unconjugated]
268-N4/CF
NT-4 [Unconjugated]
(9 Publications)
Species: Hu
Applications: BA
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(44 Publications)
Species: Rt
Applications: IHC, WB
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(217 Publications)
Species: Hu
Applications: Bind, BA
TrkC Antibody
[Unconjugated]
AF1404
Western Blot TrkC Antibody [Unconjugated]
Simple Western TrkC Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence TrkC Antibody [Unconjugated]
(1 Review)
(66 Publications)
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
TrkB Antibody
[Unconjugated]
AF1494
Immunohistochemistry TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
(12 Reviews)
(80 Publications)
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB

NGFR/TNFRSF16/p75NTR A ...
AF1157
Immunohistochemistry NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
Western Blot NGFR/TNFRSF16/p75NTR Antibody [Unconjugated]
(26 Publications)
Species: Mu
Applications: IHC, WB
tropomyosin-3
Antibody (2E4)
H00007170-M02
Sandwich ELISA tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
Western Blot tropomyosin-3 Antibody (2E4)
(2 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
CNTF [Unconjugated]
257-NT
SDS-PAGE CNTF [Unconjugated]
Bioactivity CNTF [Unconjugated]
(63 Publications)
Species: Hu
Applications: BA
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(547 Publications)
Species: Hu
Applications: BA
LEDGF Antibody
(762815) [Unconjugate
MAB3468
Western Blot LEDGF Antibody (762815) [Unconjugated]
Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC, WB
CDP/CUTL1 Antibody
(2A10)
H00001523-M01
Western Blot CDP/CUTL1 Antibody (2A10)
Immunohistochemistry-Paraffin CDP/CUTL1 Antibody (2A10)
ELISA CDP/CUTL1 Antibody (2A10)
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
TNF RII/TNFRSF1B
[HRP]
DRT200
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
N/A TNF RII/TNFRSF1B [HRP]
(76 Publications)
Species: Hu
Applications: ELISA
KHSRP Antibody
NBP1-18910
Immunohistochemistry-Paraffin KHSRP Antibody
Immunohistochemistry-Paraffin KHSRP Antibody
Western Blot KHSRP Antibody
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
HCLS1 Antibody
(1A8)
H00003059-M02
Western Blot HCLS1 Antibody (1A8)
Proximity Ligation Assay HCLS1 Antibody (1A8)
Immunohistochemistry-Paraffin HCLS1 Antibody (1A8)
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
IL-2 R beta
Antibody (27302)
MAB224
Immunohistochemistry IL-2 R beta Antibody (27302)
Neutralization IL-2 R beta Antibody (27302)
(7 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Neutralization Siglec-7/CD328 Antibody [Unconjugated]
(1 Review)
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB

Publications for NT-3 (11346-N3) (0)

There are no publications for NT-3 (11346-N3).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NT-3 (11346-N3) (0)

There are no reviews for NT-3 (11346-N3). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for NT-3 (11346-N3) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NT-3 Products

Blogs on NT-3

There are no specific blogs for NT-3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NT-3 Protein, CF and receive a gift card or discount.


Bioinformatics

Uniprot