Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

The alpha and beta forms of Neurexins 1-3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 1 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 1 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 1 beta is a 70 kDa glycosylated protein with a 313 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (2, 4, 5). Within comparable regions of the ECD, human Neurexin 1 beta shares 99% aa sequence identity with mouse and rat Neurexin 1 beta . It shares 42% and 68% aa sequence identity with the ECDs of human Neurexin 2 beta and 3 beta , respectively. Neurexin 1 beta isoforms are differentiated by a 30 aa insertion at alternative splice site 4 (SS4), numbered according to splice sites in the longer alpha Neurexins (4, 5). This recombinant protein does not include the SS4 insertion. Neurexin 1 beta is expressed presynaptically at neuronal contacts and interacts with postsynaptic Neuroligins 1-4. The presence of the SS4 insertion significantly weakens this interaction, particularly with Neuroligins 1 and 4 (6-12). Binding affinity is also dependent on the splicing pattern of the Neuroligin (7, 10, 11). Within the presynaptic terminal, Neurexin 1 beta is required for the recruitment of scaffolding proteins and synaptic vesicles (13). Its interaction with Neuroligins 1, 3, and 4 induces the development of glutamatergic postsynaptic terminals containing NMDA receptors, while its interaction with Neuroligin 2 promotes GABAergic postsynaptic development (10, 12, 14, 15). The presence of the SS4 insertion reduces the ability of Neurexin 1 beta to promote glutamatergic contacts but is required for the development of Neuroligin 2/GABAergic contact development (10, 12).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Immunoprecipitation, Neutralization.

Showing Publications 1 - 3 of 3.

Publications using 5268-NX	Applications	Species
KDB Wierda, TL Toft-Berte, CR Gøtzsche, E Pedersen, I Korshunova, J Nielsen, ML Bang, AB Kønig, S Owczarek, MD Gjørlund, M Schupp, E Bock, JB Sørensen The soluble neurexin-1&beta ectodomain causes calcium influx and augments dendritic outgrowth and synaptic transmission Sci Rep, 2020-10-22;10(1):18041. 2020-10-22 [PMID: 33093500] (Bioassay, Rat)	Bioassay	Rat
Y Kashiwagi, T Higashi, K Obashi, Y Sato, NH Komiyama, SGN Grant, S Okabe Computational geometry analysis of dendritic spines by structured illumination microscopy Nat Commun, 2019-03-20;10(1):1285. 2019-03-20 [PMID: 30894537] (Neutralization, Mouse)	Neutralization	Mouse
Y Naito, Y Tanabe, AK Lee, E Hamel, H Takahashi Amyloid-? Oligomers Interact with Neurexin and Diminish Neurexin-mediated Excitatory Presynaptic Organization Sci Rep, 2017-02-13;7(0):42548. 2017-02-13 [PMID: 28211900] (Immunoprecipitation, Human)	Immunoprecipitation	Human

Array 5268-NX

• Neurexin 1 beta/NXRN1b Antibodies
• Neurexin 1 beta/NXRN1b Proteins and Enzyme