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Recombinant Human/Mouse/Rat NT-3, Animal-Free Protein

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NT-3 bioactivity is measured using a luciferase reporter assay in HEK293T cells co-transfected with the TrkA receptor. Cells are treated in triplicate with a serial dilution of NT-3 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.EC50 = 25.4 ng/ml (0.93 nM).
NT-3 bioactivity is measured using a luciferase reporter assay in HEK293T cells co-transfected with the TrkA receptor. Cells are treated in triplicate with a serial dilution of NT-3 for 3 hours. Firefly luciferase ...read more
Human NT-3 protein (Qk058) migrates mainly as a single band at 13.7 kDa in non-reducing (NR) conditions and upon reduction (R).Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+ beta ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
Qk058-0500
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Recombinant Human/Mouse/Rat NT-3, Animal-Free Protein Summary

Additional Information
Human/Mouse/Rat/Porcine
Details of Functionality
No significant difference between EC50 of reference and test lots
Source
E. coli-derived NT-3 protein
Accession #
Protein/Peptide Type
Animal-Free Recombinant Proteins
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27.3 kDa (dimer) & 13.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Monomeric NT-3 protein only
Publications
Read Publication using
Qk058 in the following applications:

Packaging, Storage & Formulations

Storage
Store lyophilized protein between -20 and -80 °C until the date of expiry. Avoid freeze-thaw cycles.
Buffer
Lyophilized from acetonitrile/TFA
Reconstitution Instructions
Resuspend in 10 mM HCl at >100 µg/mL, prepare single use aliquots, add carrier protein if desired.

Notes

The above product was manufactured, tested and released by R&D System's contract manufacturer, Qkine Ltd, at 1 Murdoch House, Cambridge, UK, CB5 8HW. The product is for research use only and not for the diagnostic or theraputic use.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat NT-3, Animal-Free Protein

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for NT-3 (Qk058)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Cell Culture.


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