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Recombinant Human Kallikrein 8/Neuropsin Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

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Recombinant Human Kallikrein 8/Neuropsin Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011). The specific activity is >500 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 8/Neuropsin protein
Gln29-Gly260, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results obtained: Gln29 predicted
Structure / Form
Pro form
Protein/Peptide Type
Recombinant Enzymes
Gene
KLK8
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
37-39 kDa, reducing conditions
Publications
Read Publications using
2025-SE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Activation Buffer: 50 mM Tris, pH 8.0
  • Assay Buffer: 50 mM Tris, pH 9.0
  • Recombinant Human Kallikrein 8/Neuropsin (rhKLK8) (Catalog # 2025-SE)
  • Lysyl-Endopeptidase, 20 U/mL stock in PBS
  • Substrate: BOC-Val-Pro-Arg-AMC (Catalog # ES011), 10 mM stock in DMSO
  • Black 96-well Plate
  • Plate Reader with Fluorescence Read Capability
  1. Dilute rhKLK8 to 200 µg/mL in Activation Buffer.
  2. Dilute Lysyl-Endopeptidase to 0.4 mU/mL in Activation Buffer.
  3. Activate rhKLK8 by combining equal volumes of diluted rhKLK8 and Lysyl-Endopeptidase.
  4. Incubate at 37 °C for 30 minutes.
  5. Dilute activated rhKLK8 to 1 ng/µL in Assay Buffer.
  6. Dilute Substrate to 200 µM in Assay Buffer.
  7. Load 50 µL of 1 ng/µL rhKLK8 into the plate, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 200 µM Substrate.
  8. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively in kinetic mode for 5 minutes.
  9. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC).

Per Well:
  • rhKLK8: 0.050 µg
  • Substrate: 100 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Kallikrein 8/Neuropsin Protein, CF

  • EC 3.4.21
  • EC 3.4.21.118
  • hK8
  • HNP
  • kallikrein 8 (neuropsin/ovasin)
  • Kallikrein 8
  • kallikrein-related peptidase 8
  • KLK8 protein type 1
  • KLK8 protein type 2
  • KLK8
  • neuropsin type 1
  • neuropsin type 2
  • Neuropsin
  • NP
  • NRPN
  • Ovasin
  • PRSS19
  • PRSS19kallikrein-8
  • Serine protease 19
  • Serine protease TADG-14
  • TADG14neuropsin
  • Tumor-associated differentially expressed gene 14 protein

Background

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (residues 29 to 32) and the mature chain (residues 33 to 260). Isoform 2 is identical to isoform 1, except that a 45 amino acid segment is inserted in isoform 2 between residues 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8). This activity can be inhibited by recombinant human Serpin A5, recombinant human Serpin F2 and AEBSF (Catalog # 1266-PI, 1470-PI and EI001, respectively). Recombinant human KLK8 produced by R&D Systems corresponds to isoform 1.

  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
  3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
  4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
  5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
  6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
  7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
  8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.

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Recombinant Human
Kallikrein 8/Neuropsi ...
2025-SE
Recombinant Human Kallikrein 8/Neuropsin Protein, CF
Species: Hu
Applications: Enzyme Activity

Publications for Kallikrein 8/Neuropsin (2025-SE)(6)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, Enzyme Assay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
Enzyme Assay
(3)
In Vivo
(1)
Surface Plasmon Resonance
(1)
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Human
(3)
Mouse
(1)
N/A
(1)
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Showing Publications 1 - 6 of 6.
Publications using 2025-SE Applications Species
E Bosc, J Anastasie, F Soualmia, P Coric, JY Kim, LQ Wang, G Lacin, K Zhao, R Patel, E Duplus, P Tixador, AA Sproul, B Brugg, M Reboud-Rav, CM Troy, ML Shelanski, S Bouaziz, M Karin, C El Amri, ED Jacotot Genuine selective caspase-2 inhibition with new irreversible small peptidomimetics Cell Death & Disease, 2022-11-15;13(11):959. 2022-11-15 [PMID: 36379916] (Bioassay, N/A) Bioassay N/A
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Neuropsin cleaves EphB2 in the amygdala to control anxiety. Nature, 2011-04-20;473(7347):372-5. 2011-04-20 [PMID: 21508957] (Bioassay, Enzyme Assay, In Vivo, Human, Mouse) Bioassay, Enzyme Assay, In Vivo Human, Mouse
Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder J, Meyer-Hoffert U Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides, 2011-03-23;32(6):1187-92. 2011-03-23 [PMID: 21439340] (Bioassay) Bioassay
Meyer-Hoffert U, Wu Z, Kantyka T, Fischer J, Latendorf T, Hansmann B, Bartels J, He Y, Glaser R, Schroder JM Isolation of SPINK6 in human skin: selective inhibitor of kallikrein-related peptidases. J. Biol. Chem., 2010-07-28;285(42):32174-81. 2010-07-28 [PMID: 20667819] (Enzyme Assay, Human) Enzyme Assay Human
Sexton DJ, Chen T, Martik D, Kuzmic P, Kuang G, Chen J, Nixon AE, Zuraw BL, Forteza RM, Abraham WM, Wood CR Specific inhibition of tissue kallikrein 1 with a human monoclonal antibody reveals a potential role in airway diseases. Biochem. J., 2009-08-13;422(2):383-92. 2009-08-13 [PMID: 19527222] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Yoon H, Laxmikanthan G, Lee J, Blaber SI, Rodriguez A, Kogot JM, Scarisbrick IA, Blaber M Activation profiles and regulatory cascades of the human kallikrein-related peptidases. J. Biol. Chem., 2007-09-06;282(44):31852-64. 2007-09-06 [PMID: 17823117] (Enzyme Assay) Enzyme Assay

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Bioinformatics

Gene Symbol KLK8
Uniprot