| Reactivity | HuSpecies Glossary |
| Applications | Bioactivity |
| Format | Carrier-Free |
| Additional Information | Soon to be discontinued. |
| Details of Functionality | |
| Source | Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP10 protein Met1 - Leu798 with a C-terminal 6-His tag |
| Accession # | |
| Protein/Peptide Type | Recombinant Enzymes |
| Gene | USP10 |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
| Dilutions |
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| Theoretical MW | 89 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Supplied as a solution in HEPES, NaCl, TCEP, and Glycerol. |
| Purity | >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a specialized cysteine protease with a predicted molecular weight of 87 kDa. USP10 is a member of the peptidase C19 family and the human protein shares 83% amino acid sequence identity with its mouse ortholog. Reported USP10 targets include p53, BECN1, SNX3 and CFTR. USP10 translocates to the nucleus in response to DNA damage and deubiquitinates p53, thus regulating the p53-dependent DNA damage response. USP10 deubiquitination of BECN1 leads to stabilization of PIK3C3/VPS34-containing complexes which drives autophagy. The small molecule Spautin-1 exerts its potent inhibitory effects on autophagy partially through the inhibition of USP10 activity.
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Beclin 1: Regulator of Autophagy and Apoptosis Beclin 1 is the mammalian orthologue of the yeast Apg6/Vps30 gene. Beclin 1 can complement the defect in autophagy present in apg6 yeast strains and stimulate autophagy when overexpressed in mammalian cells (1) and can bind to Bcl2, an important regul... Read full blog post. |
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