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Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
368-ST-050/CF
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Recombinant Human Flt-3/Flk-2 Fc Chimera Protein Summary

Details of Functionality
Measured by its ability to inhibit Flt-3 Ligand-induced proliferation of BaF3 mouse pro‑B cells transfected with Flt-3. The ED50 for this effect is 0.01-0.03 µg/mL in the presence of rhFlt-3 Ligand at 2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Flt-3/Flk-2 protein
Human Flt-3
(Asn27-Asn541)
Accession # AAA18947		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asn27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FLT3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
85.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
368-ST in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Flt-3/Flk-2 Fc Chimera Protein

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Human Flt-3 cDNA encodes a 993 amino acid (aa) residue type I membrane protein with a 26 aa residue signal peptide, a 515 aa extracellular domain with 10 potential N-linked glycosylation sites, a 21 aa residue transmembrane domain and a 431 aa residue cytoplasmic domain. Mouse Flt-3 has also been cloned and shown to share 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin. Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3/Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist.

  1. Rosnet, O. et al. (1996) Acta. Haemato. 95:218.
  2. Drexler, H.G. (1996) Leukemia 10:588.

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Publications for Flt-3/Flk-2/CD135 (368-ST)(5)

We have publications tested in 2 confirmed species: Human, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Bioassay, Cell Culture, SPR.


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Bioassay
(4)
Cell Culture
(1)
SPR
(1)
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Human
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 5 of 5.
Publications using 368-ST Applications Species
A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain Progress in neurobiology, 2023-01-13;0(0):102405. 2023-01-13 [PMID: 36646299] (Bioassay, SPR, Human) Bioassay, SPR Human
Q Jiang, J Isquith, L Ladel, A Mark, F Holm, C Mason, Y He, P Mondala, I Oliver, J Pham, W Ma, E Reynoso, S Ali, IJ Morris, R Diep, C Nasamran, G Xu, R Sasik, SB Rosenthal, A Birmingham, S Coso, G Pineda, L Crews, ME Donohoe, JC Venter, T Whisenant, RA Mesa, LB Alexandrov, KM Fisch, C Jamieson Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution Cell Reports, 2021-01-26;34(4):108670. 2021-01-26 [PMID: 33503434] (Bioassay, Human) Bioassay Human
MA Kinney, LT Vo, JM Frame, J Barragan, AJ Conway, S Li, KK Wong, JJ Collins, P Cahan, TE North, DA Lauffenbur, GQ Daley A systems biology pipeline identifies regulatory networks for stem cell engineering Nat. Biotechnol., 2019-07-01;37(7):810-818. 2019-07-01 [PMID: 31267104] (Cell Culture, Human) Cell Culture Human
Y Heshmati, S Kharazi, G Türköz, D Chang, E Kamali Dol, J Boström, A Krstic, T Boukoura, E Wagner, N Kadri, R Månsson, M Altun, H Qian, J Walfridsso The histone chaperone NAP1L3 is required for haematopoietic stem cell maintenance and differentiation Sci Rep, 2018-07-25;8(1):11202. 2018-07-25 [PMID: 30046127] (Bioassay, Human) Bioassay Human
Donahue RE, Wersto RP, Allay JA, Agricola BA, Metzger ME, Nienhuis AW, Persons DA, Sorrentino BP High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34(+) cells. Blood, 2000-01-15;95(2):445-52. 2000-01-15 [PMID: 10627448] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)

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Bioinformatics

Gene Symbol FLT3
Uniprot