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Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

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When Recombinant Human FCRL4 (Catalog # 2426-FC) is coatedonto a microplate at 0.5 µg/mL, Biotinylated Human IgA binds with an ED50 of5‑20 μg/mL.
When Recombinant Human FCRL4 (Catalog # 2426-FC) is coatedonto a microplate at 0.5 µg/mL, Biotinylated Human IgA binds with an ED50 of5‑20 μg/mL.
2 μg/lane of Recombinant Human FCRL4/FcRH4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 53-60 kDa and 45-52 kDa, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human FCRL4/FcRH4 His-tag Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human FCRL4/FcRH4 is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Human IgA binds with an ED50 of 5‑20 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human FCRL4/FcRH4 protein
Ala18-Arg385, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala18
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
53-60 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

  • CD307d antigen
  • CD307d
  • Fc receptor homolog 4
  • Fc receptor-like 4
  • FcRH4
  • FCRH4Fc receptor-like protein 4
  • FCRL4
  • FcR-like protein 4
  • hIFGP2
  • IFGP family protein 2
  • IFGP2
  • IGFP2
  • Immune receptor translocation-associated protein 1
  • immunoglobulin superfamily Fc receptor, gp42
  • immunoglobulin superfamily receptor translocation associated 1
  • IRTA1
  • IRTA1MGC150523
  • MGC150522

Background

Fc receptor-like 4 (FCRL4), also known as FcRH4, IFGP2 and IRTA1, is a member of the Ig superfamily transmembrane protein, which is preferentially expressed in B cells and contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1). Mature human FCRL4 consists of a 368 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-type domains, a 21 aa transmembrane segment, and a 107 aa cytoplasmic domain with three ITIM-like motifs. FCRL4 is closely related to FC gamma RIIB (2, 3) and shows high homology to Fc receptors (4). In cellular binding assays, FCRL4 bound to IgA efficiently (5). The FCRL4 gene is localized in the human chromosome 1q21-23 region, a hotspot for translocation events (3). The B cells expressing FCRL4 participate in the autoimmune response in rheumatoid arthritis patients (6).
  1. Ehrhardt, G.R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:13489.
  2. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. U. S. A. 98:9772.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. Amara, K. et al. (2007) J. Autoimmun. 81:34.
  5. Wilson, T.J. et al. (2012) J. Immunol. 188:4741.
  6. Miller, I. et al. (2002) Blood 99:2662.

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Recombinant Human FCRL4/FcRH4 His-tag Protein, CF
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