Recombinant Human EphA5 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1‑4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54‑61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, Control, ELISA Capture, Immunization.

Showing Publications 1 - 4 of 4.

Publications using 3036-A5	Applications	Species
JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-03-10;0(0):101817. 2022-03-10 [PMID: 35278434] (ELISA Capture, N/A)	ELISA Capture	N/A
S D'Angelo, FI Staquicini, F Ferrara, DI Staquicini, G Sharma, CA Tarleton, H Nguyen, LA Naranjo, RL Sidman, W Arap, AR Bradbury, R Pasqualini Selection of phage-displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications JCI Insight, 2018-05-03;3(9):. 2018-05-03 [PMID: 29720567] (Bioassay, Human)	Bioassay	Human
Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, Moeller B, Kelly P, Nunez M, Tang X, Liu D, Lee J, Hong W, Ferrara F, Bradbury A, Lobb R, Edelman M, Sidman R, Wistuba I, Arap W, Pasqualini R Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. J Biol Chem, 2015-01-26;290(12):7345-59. 2015-01-26 [PMID: 25623065] (Immunization, Mouse)	Immunization	Mouse
Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi's sarcoma-associated herpesvirus. Nat. Med., 2012-06-01;18(6):961-6. 2012-06-01 [PMID: 22635007] (Control, Human)	Control	Human

Array 3036-A5

• EphA5 Antibodies
• EphA5 Proteins and Enzymes