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Frk Antibody (OTI6D2)

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Western Blot: Frk Antibody (OTI6D2) [NBP1-47761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.
Flow Cytometry: Frk Antibody (OTI6D2) [NBP1-47761] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FRK ...read more
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Frk Antibody (OTI6D2) [NBP1-47761] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI6D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Frk Antibody (OTI6D2) Summary

Immunogen
Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens fyn-related kinase (FRK).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FRK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
Theoretical MW
58.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Frk Antibody (OTI6D2)

  • EC 2.7.10
  • Frk
  • fyn-related kinaseProtein-tyrosine kinase 5
  • Gtk
  • GTKEC 2.7.10.2
  • Nuclear tyrosine protein kinase RAK
  • PTK5 protein tyrosine kinase 5
  • PTK5
  • Rak
  • RAKPTK5
  • tyrosine-protein kinase FRK

Background

The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FRK
Entrez