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Recombinant Human EBI3 Protein, CF

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Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein ...read more
2 μg/lane of Recombinant Human EBI3 Protein (Catalog # 11084-EI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human EBI3 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human EBI3 Protein (Catalog # 11084-EI) is immobilized at 5.0 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL-27 p28/IL-30 Protein (Catalog # 7430-ML) that produces 50% of the optimal binding response is 40.0-300 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human EBI3 protein
Arg21-Lys220
Accession #
N-terminal Sequence
Arg21
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-40 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EBI3 Protein, CF

  • cytokine receptor
  • EBI3
  • EBI-3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 beta
  • IL-27 subunit beta
  • IL27B
  • IL-27B
  • IL35 EBI3
  • IL-35 EBI3
  • interleukin-27 subunit beta

Background

EBI3 (Epstein-Barr virus-induced gene 3 protein) also known as IL-27B, is a secreted cytokine (1). It shares similar domain structure as IL-12B except there are only two fibronectin type -III domains instead of three. Through non-covalent interaction, EBI-3 binds to IL-12A (IL-12 p35), IL-23A (IL-23p19), or IL-27A (IL-27p28/IL-30) to form IL-35, IL-39, or IL-27, respectively (2-4). In addition to forming the heterodimeric cytokines, EBI-3 can also function on its own. Expression of EBI-3 is induced in neutrophils stimulated with TLR8 ligands, with no detectable increase in IL-35, IL-39 or IL-27 (5). EBI-3 also mediate IL-6 trans-signaling through receptor gp130 in B9 mouse plasmacytoma cells (6).
  1. Devergne, O. et al. (1996) J. Virol. 70:1143.
  2. Devergne, O. et al. (1997) Protc. Natl. Acad. Sci. U.S.A. 94:12041.
  3. Pflanz, S. et al. (2002) Immunity 16:779.
  4. Wang, X. et al. (2016) Eur. J. Immuno. 46:1343.
  5. Cassatella, M.A. et al. (2020) J Leukoc. Biol. 108:1515.
  6. Chehboun, S. et al. (2017) J Biol. Chem. 292:6644.

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Recombinant Human
EBI3 Protein, CF
11084-EI
Recombinant Human EBI3 Protein, CF
Species: Hu
Applications: Bioactivity

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