Recombinant Human Contactin-3 Protein, CF Summary
Details of Functionality |
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the recombinant human Contactin-2 solution at 6-24 μg/mL. |
Source |
Mouse myeloma cell line, NS0-derived human Contactin-3 protein Glu20-Pro992, with a C-terminal 10-His tag |
Accession # |
|
N-terminal Sequence |
Glu20 |
Protein/Peptide Type |
Recombinant Proteins |
Gene |
CNTN3 |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
108.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
140-150 kDa, reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions |
Reconstitute at 300 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Contactin-3 Protein, CF
Background
Contactin-3, also known as BIG-1 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).
- Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
- Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
- Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
- Yoshihara, Y. et al. (1994) Neuron 13:415.
- Kamei, Y. et al. (2000) Genomics 69:113.
- Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.
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