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Recombinant Human Contactin-3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Contactin-3 Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96-well culture plates that had been pre-coated with 50 µL/well of the recombinant human Contactin-2 solution at 6-24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Contactin-3 protein
Glu20-Pro992, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Glu20
Protein/Peptide Type
Recombinant Proteins
Gene
CNTN3
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
108.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
140-150 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-3 Protein, CF

  • BIG-1
  • CNTN3
  • contactin 3 (plasmacytoma associated)
  • Contactin3
  • Contactin-3
  • PANG
  • PCS

Background

Contactin-3, also known as BIG-1 and PANG, is a 150 kDa member of the TAG/F3 subgroup of Contactin neuronal adhesion proteins (1, 2). Mature human Contactin-3 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3, 4). Human Contactin-3 shares 92% aa sequence identity with mouse and rat Contactin-3. Contactin-3 is primarily expressed in the brain in discrete regions including the frontal lobe, occipital lobe, cerebellum, amygdala, and hippocampus (5, 6). Alternative splicing in rat may generate a soluble isoform (4). Mouse plasmacytoma cell lines produce multiple transcripts of Contactin-3 due to the presence of IAP proviruses (3). Immobilized Contactin-3 promotes neurite outgrowth from rat hippocampal neurons (4).
  1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
  2. Denisenko-Nehrbass, N. et al. (2002) J. Physiol. 96:99.
  3. Connelly, M.A. et al. (1994) Proc. Natl. Acad. Sci. 91:1337.
  4. Yoshihara, Y. et al. (1994) Neuron 13:415.
  5. Kamei, Y. et al. (2000) Genomics 69:113.
  6. Yoshihara, Y. et al. (1995) J. Neurobiol. 28:51.

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Bioinformatics

Gene Symbol CNTN3
Uniprot