Novus Biologicals products are now on bio-techne.com

Recombinant Human Complement Component C5a Protein, CF

Images

 
There are currently no images for Complement Component C5a (2037-C5/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human Complement Component C5a Protein, CF Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 1.5-5 ng/mL.
Source
E. coli-derived human Complement Component C5a protein
Thr678-Arg751
Accession #
N-terminal Sequence
Thr678
Protein/Peptide Type
Recombinant Proteins
Gene
C5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2037-C5/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Component C5a Protein, CF

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

  1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Customers Who Viewed This Item Also Viewed...

NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-91803
Species: Hu
Applications: IHC, IHC-P, WB
H00005688-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
NBP3-12295
Species: Hu, Pm, Rt
Applications: ELISA, IHC, IHC-P, IP, WB
NBP2-36776
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
MAB3648
Species: Hu
Applications: CyTOF-ready, Flow, Neut
AF1936
Species: Hu
Applications: IP, WB
H00000730-D01P
Species: Hu, Mu
Applications: WB
DIP100
Species: Hu
Applications: ELISA
NBP1-88527
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-93935
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP2-15952
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
MAB6529
Species: Hu
Applications: Simple Western, WB
NBP3-12295
Species: Hu, Pm, Rt
Applications: ELISA, IHC, IHC-P, IP, WB
NBP3-16514
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-89985
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP1-87492
Species: Hu
Applications: IHC, IHC-P, WB
2037-C5/CF
Species: Hu
Applications: Bioactivity

Publications for Complement Component C5a (2037-C5/CF)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, Hemofiltration Procedure, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(17)
Cell Culture
(2)
Hemofiltration Procedure
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(19)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using 2037-C5/CF Applications Species
AJ Plant-Hate, B Eryilmaz, CAW David, DE Brain, BJ Heaton, Y Perrie, NJ Liptrott Exposure of the Basophilic Cell Line KU812 to Liposomes Reveals Activation Profiles Associated with Potential Anaphylactic Responses Linked to Physico-Chemical Characteristics Pharmaceutics, 2022-11-15;14(11):. 2022-11-15 [PMID: 36432660] (Bioassay, Human) Bioassay Human
J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation BMC Oral Health, 2021-09-24;21(1):470. 2021-09-24 [PMID: 34560867] (Bioassay, Human) Bioassay Human
D Medica, R Franzin, A Stasi, G Castellano, M Migliori, V Panichi, F Figliolini, L Gesualdo, G Camussi, V Cantaluppi Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury Cells, 2021-07-02;10(7):. 2021-07-02 [PMID: 34359843] (Bioassay, Human) Bioassay Human
S Silawal, B Kohl, J Shi, G Schulze-Ta Complement Regulation in Human Tenocytes under the Influence of Anaphylatoxin C5a International Journal of Molecular Sciences, 2021-03-18;22(6):. 2021-03-18 [PMID: 33803624] (Bioassay, Human) Bioassay Human
N Kashiwagi, F Saito, H Maegawa, K Kaneda Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results Cytokine, 2021-01-01;139(0):155410. 2021-01-01 [PMID: 33395614] (Bioassay, Human) Bioassay Human
L Hong, Y Tang, S Pan, M Xu, Y Shi, S Gao, C Sui, C He, K Zheng, R Tang, Z Shi, Q Wang, H Wang Interleukin 3-induced GITR promotes the activation of human basophils Cytokine, 2020-09-02;136(0):155268. 2020-09-02 [PMID: 32889153] (Bioassay, Human) Bioassay Human
S Ohlsson, L Holm, C Hansson, SM Ohlsson, L Gunnarsson, Å Pettersson, L Skattum Neutrophils from ANCA-associated vasculitis patients show an increased capacity to activate the complement system via the alternative pathway after ANCA stimulation PLoS ONE, 2019-06-19;14(6):e0218272. 2019-06-19 [PMID: 31216309] (Bioassay, Cell Culture, Human) Bioassay, Cell Culture Human
J Paupert, E Espinosa, N Cenac, V Robert, P Laharrague, SM Evrard, L Casteilla, A Lorsignol, B Cousin Rapid and Efficient Production of Human Functional Mast Cells Through a Three-Dimensional Culture of Adipose Tissue-Derived Stromal Vascular Cells J. Immunol., 2018-11-16;0(0):. 2018-11-16 [PMID: 30446570] (Cell Culture, Human) Cell Culture Human
Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR Biosci. Rep., 2018-05-15;0(0):. 2018-05-15 [PMID: 29685955] (Bioassay, Human) Bioassay Human
MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018-01-25;13(1):e0191676. 2018-01-25 [PMID: 29370247] (Bioassay, Human) Bioassay Human
Show All 21 Publications.

Reviews for Complement Component C5a (2037-C5/CF) (0)

There are no reviews for Complement Component C5a (2037-C5/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Complement Component C5a (2037-C5/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Complement Component C5a Products

Blogs on Complement Component C5a

There are no specific blogs for Complement Component C5a, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Complement Component C5a Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol C5
Uniprot