Novus Biologicals products are now on bio-techne.com

RBM3 Antibody

Images

 
Genetic Strategies: Western Blot: RBM3 Antibody [NBP1-81580] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: RBM3 Antibody [NBP1-81580] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Western Blot: RBM3 Antibody [NBP1-81580] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human breast cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human colorectal cancer shows weak nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: RBM3 Antibody [NBP1-81580] - Staining of human kidney shows no nuclear positivity in cells in glomeruli as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

RBM3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DEQALEDHFSSFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAMNGESLDGRQIRVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGYGYGRSRDYNGRNQGGYDRYSGGNY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RBM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RBM3 Protein (NBP1-81580PEP)
Publications
Read Publications using NBP1-81580.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 21898641)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RBM3 Antibody

  • IS1-RNPL
  • putative RNA-binding protein 3
  • RNA binding motif (RNP1, RRM) protein 3
  • RNA binding motif protein 3
  • RNA-binding motif protein 3
  • RNPL

Background

RBM3 is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-15745
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
DVE00
Species: Hu
Applications: ELISA
NBP3-13106
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-124
Species: Bv, Ma, Hu, Mu, Pm, Rt, Sh
Applications: ChIP, CHIP-SEQ, GS, IB, ICC/IF, IHC, IHC-P, IP, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NB100-105
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NBP1-84951
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP3-27724
Species: Hu
Applications: ICC/IF, IHC, WB
NB100-464
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP1-71691
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32887
Species: Hu
Applications: IHC, IHC-P, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-81580
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, KD

Publications for RBM3 Antibody (NBP1-81580)(3)

Reviews for RBM3 Antibody (NBP1-81580) (0)

There are no reviews for RBM3 Antibody (NBP1-81580). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RBM3 Antibody (NBP1-81580) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RBM3 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RBM3