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RAR gamma/NR1B3 Overexpression Lysate

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Western Blot: Retinoic Acid Receptor gamma Overexpression Lysate (Adult Normal) [NBL1-15159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Retinoic Acid Receptor gamma.
Western Blot: Retinoic Acid Receptor gamma Overexpression Lysate (Adult Normal) [NBL1-15159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Retinoic Acid ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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RAR gamma/NR1B3 Overexpression Lysate Summary

Description

RAR gamma/NR1B3 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210815

Accession#: NM_000966

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
RARG

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
50.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RAR gamma/NR1B3 Overexpression Lysate

  • NR1B3
  • NR1B3RARC
  • Nuclear receptor subfamily 1 group B member 3
  • RAR gamma
  • RARC
  • RARG
  • RAR-gamma
  • retinoic acid receptor gamma
  • retinoic acid receptor, gamma

Background

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters. Retinoic acid receptor gamma has been shown to be the most important retinoid receptor for regulation of retinoic acid turnover rate and retinoic acid induced growth inhibition. While RAR mRNA levels may be useful biomarkers for various diseases, RAR gamma expression is decreased in Barrett's tissues compared with normal tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RARG