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PSMC3 Antibody

Images

 
Western Blot: PSMC3 Antibody [NBP1-86962] - Analysis of human fibroblasts. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody [NBP1-86962] - Staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-86962] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Western Blot: PSMC3 Antibody [NBP1-86962] - Analysis in human cell line MCF-7.
Western Blot: PSMC3 Antibody [NBP1-86962] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Simple Western: PSMC3 Antibody [NBP1-86962] - Mouse whole-spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this PSMC3 antibody. Simple Western image submitted by a verified customer ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PSMC3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMC3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western Validated from a verified customer review
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PSMC3 Protein (NBP1-86962PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-86962 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PSMC3 Antibody

  • human immunodeficiency virus tat transactivator binding protein-1
  • MGC8487
  • proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5
  • Proteasome 26S subunit ATPase 3
  • Proteasome subunit P50
  • TBP-126S protease regulatory subunit 6A
  • TBP1Tat-binding protein 1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PSMC3 Antibody (NBP1-86962) (0)

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Reviews for PSMC3 Antibody (NBP1-86962) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-86962:
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Simple Western
(1)
WB
(1)
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Human
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Images Ratings Applications Species Date Details
Simple Western PSMC3 NBP1-86962
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4
reviewed by:
Verified Customer
Simple Western Mouse 08/23/2019
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Summary

ApplicationSimple Western
Sample TestedMouse Spleen Lysate
SpeciesMouse
LotA90016
Western Blot PSMC3 NBP1-86962
Enlarge
4
reviewed by:
Verified Customer
WB Human 04/19/2018
View

Summary

ApplicationWestern Blot
Sample Testedfibroblasts
SpeciesHuman
LotA90016

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMC3 Antibody (NBP1-86962) (0)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
08/23/2019
Application: Simple Western
Species: Mouse

Verified Customer
04/19/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol PSMC3