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Human PSAP - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human PSAP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38986] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, seminal plasma, cell culture supernatants and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.055 ng/mL (example only; lot dependent)

Order Details

Human PSAP - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.055 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PSAP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human PSAP - Ready-To-Use ELISA Kit (Colorimetric)

  • FLJ00245
  • GLBA
  • proactivator polypeptide
  • prosaposin
  • PSAP
  • SAP1
  • SAP1MGC110993
  • sphingolipid activator protein-1

Background

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D, which are similar to each other and are sphingolipid hydrolase activator proteins. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in PSAP gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PSAP