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PRPF8 Antibody (4I1Q8)

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Western Blot: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Western blot analysis of extracts of various cell lines, using PRPF8 Rabbit mAb (NBP3-16440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunofluorescence analysis of U-2 OS cells using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunohistochemistry of paraffin-embedded mouse spinal cord using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens).Perform microwave ...read more
Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunohistochemistry of paraffin-embedded rat brain using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: PRPF8 Antibody (4I1Q8) [NBP3-16440] - Immunohistochemistry of paraffin-embedded human colon using PRPF8 Rabbit mAb (NBP3-16440) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4I1Q8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PRPF8 Antibody (4I1Q8) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2335 of human PRPF8 (Q6P2Q9). MADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRPF8
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PRPF8 Antibody (4I1Q8)

  • apoptosis-regulated protein 1
  • apoptosis-regulated protein 2
  • p220
  • precursor mRNA processing protein
  • pre-mRNA-processing-splicing factor 8
  • PRP8 homolog
  • PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
  • PRP8 pre-mRNA processing factor 8 homolog (yeast)
  • Prp8
  • PRP8,220 kDa U5 snRNP-specific protein
  • PRPC8HPRP8
  • RP13
  • Splicing factor Prp8
  • U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

Background

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PRPF8 Products

Array NBP3-16440

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Bioinformatics

Gene Symbol PRPF8