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Prostatic Acid Phosphatase/ACPP Antibody

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Western Blot: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of Human Prostate lysate with antibody at 0.01 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: Prostatic Acid Phosphatase/ACPP Antibody [NBP1-78280] - Staining of paraffin embedded Human Prostate with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Prostatic Acid Phosphatase/ACPP Antibody Summary

Immunogen
Peptide with sequence C-NHMKRATQIPSYKK corresponding to internal region according to NP_001127666.1, NP_001090.2.
Epitope
C-NHMKRATQIPSYKK,
Specificity
This antibody is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ACP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 50 kDa band observed in human prostate lysates (calculated MW of 48.3 kDa band according to NP_001127666.1). IHC-P: Human prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland.
Publications
Read Publication using NBP1-78280.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Prostatic Acid Phosphatase/ACPP Antibody

  • acid phosphatase, prostate
  • ACP3
  • ACP-3
  • ACPP
  • EC 3.1.3.2
  • PAP
  • Prostatic Acid Phosphatase
  • prostatic acid phosphotase

Background

Prostatic Acid Phosphatase encodes an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and secreted by the epithelial cells of the prostrate gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prostatic Acid Phosphatase/ACPP Antibody (NBP1-78280)(1)


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Bioinformatics

Gene Symbol ACP3
Uniprot