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Prostasin/Prss8 Antibody (530622)

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Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostasin/Prss8 at approximately 40 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more
Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) at 15 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
530622
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB4599-SP
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Prostasin/Prss8 Antibody (530622) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Prostasin/Prss8
Ala33-Gly319
Accession # Q16651
Specificity
Detects human Prostasin/Prss8 in direct ELISAs and Western blots.  In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Prostasin/Prss8 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRSS8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostasin/Prss8 Antibody (530622)

  • CAP1
  • channel-activating protease 1
  • EC 3.4.21
  • EC 3.4.21.-
  • EC 3.4.21.120
  • Prostasin
  • protease, serine, 8
  • Prss8
  • Serine protease 8

Background

Prostasin, also known as Prss8 and channel activating protease 1, is a 40 kDa serine protease with trypsin-like substrate specificity that is a member of the peptidase S1 family. The enzyme is synthesized from a 343 amino acid (aa) preproenzyme that contains a 29 aa signal peptide, a propeptide (aa 30-32) and a 290 aa mature chain. A second propeptide (aa 323-343) contains a 21 aa transmembrane region (GPI anchor) which can be proteolytically processed to generate a secreted form of the enzyme. The mature chain forms a heterodimer made up of a light chain (aa 33-44) and a heavy chain (aa 45-322) that are connected by disulfide bonds. In addition, aa 45-286 constitute a peptidase S1 domain, and aa 159 is a potential site for N-linked glycosylation. Mature human Prostasin is 79% and 78% aa identical to mature mouse and rat Prostasin, respectively. Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, salivary gland, and pancreas. It activates amiloride-sensitive sodium channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Prostasin/Prss8
Antibody (530622)
MAB4599
Prostasin/Prss8 Antibody (530622)
Species: Hu
Applications: WB, IHC, IP

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Bioinformatics

Gene Symbol PRSS8
Uniprot