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Prolyl Oligopeptidase/PREP Antibody

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Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: Prolyl Oligopeptidase/PREP Antibody [NB100-40792] - staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Prolyl Oligopeptidase/PREP Antibody Summary

Immunogen
Peptide with sequence C-QYIVGRSRKQSNP corresponding to internal region according to NP_002717.3.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PREP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human brain, lymph node, spleen and testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7 kDa band according to NP_002717.3. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows distinct staining patterns in the cytoplam of PCT.
Control
Prolyl Oligopeptidase/PREP Overexpression Lysate
Publications
Read Publication using NB100-40792.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Prolyl Oligopeptidase/PREP Antibody

  • dJ355L5.1 (prolyl endopeptidase)
  • EC 3.4.21.26
  • MGC16060
  • PE
  • PEP
  • Post-proline cleaving enzyme
  • PREP
  • prolyl endopeptidase
  • Prolyl Oligopeptidase
  • rPop

Background

The protein encoded by the Prolyl Endopeptidase gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolyl Oligopeptidase/PREP Antibody (NB100-40792)(1)

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Bioinformatics

Gene Symbol PREP
Entrez
Uniprot