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Prolactin R Antibody (U5) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Eq, Ha, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
U5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

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Prolactin R Antibody (U5) [Janelia Fluor® 635] Summary

Immunogen
Purified rat liver PRL receptor.
Epitope
This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain.
Localization
Cytoplasmic
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRLR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 2707162). Bovine reactivity reported in scientific literature (PMID: 25212395). Mouse reactivity reported in scientific literature (PMID: 19211889). Hamster reactivity reported in scientific literature (PMID: 24142695). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Prolactin R Antibody (U5) [Janelia Fluor® 635]

  • delta 4-delta 7/11 truncated prolactin receptor
  • delta 4-SF1b truncated prolactin receptor
  • hPRL receptor
  • hPRLrI
  • PRLR
  • PRL-R
  • Prolactin R
  • prolactin receptor delta 7/11
  • prolactin receptor
  • ProlactinR
  • secreted prolactin binding protein

Background

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRLR