Prolactin R Antibody (T6) - Azide and BSA Free Summary
Immunogen |
Purified rat liver PRL receptor. |
Specificity |
Detects prolactin (PRL) receptor in rat tissues. This does not cross-react with Growth Hormone (GH) receptor. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
PRLR |
Purity |
Protein A purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:10-1:500
- Immunohistochemistry 1:25 - 1:200
- Immunohistochemistry-Paraffin 1:25 - 1:200
- Western Blot 1 ug/ml
|
Application Notes |
WB: Detects an approx. 42 kDa protein representing PRL receptor in NB2 cell lysate. |
Publications |
|
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
Protein A purified |
Reconstitution Instructions |
Reconstitute with 0.1 ml PBS. |
Alternate Names for Prolactin R Antibody (T6) - Azide and BSA Free
Background
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
Species: Hu, Pm, Mu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, WB
Species: Hu, Mu
Applications: ChIP, Simple Western, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Mu
Applications: CyTOF-ready, ICC, IP, ICFlow, KO, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: BA
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: ICC, Simple Western, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu, Mu
Applications: WB
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Species: Rt
Applications: WB, ICC/IF, IHC
Publications for Prolactin R Antibody (NB300-531)(7)
Showing Publications 1 -
7 of 7.
Publications using NB300-531 |
Applications |
Species |
Aleman-Garcia YP, Vaquero-Garcia RM, Flores-Fernandez R et al. Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice Journal of immunology research 2021-03-08 [PMID: 33763492] |
|
|
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol. 1993 Jun 15;150(12):5673-81. 1993-01-01 [PMID: 8515082] |
|
|
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endo., 134(5): 2108-2114. 1994-01-01 [PMID: 8156910] |
|
|
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endo., 135(4): 1299-1306. 1994-01-01 [PMID: 7925093] |
|
|
Okamura H, Zachwieja J, Raguet S, Kelly PA. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endo., 124(5): 2499-2508. 1989-01-01 [PMID: 2707162] |
|
|
Dardenne M, Kelly PA, Bach JF, Savino W. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc. Natl. Acad. Sci., USA, 88: 9700-9704. 1991-01-01 [PMID: 1946390] |
|
|
Rozakis-Adcock M, Kelly PA. Mutational analysis of the ligand-binding domain of the prolactin receptor. J. Biol. Chem., 266(25): 16472-16477. 1991-01-01 [PMID: 1885580] |
|
|
Reviews for Prolactin R Antibody (NB300-531) (0)
There are no reviews for Prolactin R Antibody (NB300-531).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Prolactin R Antibody (NB300-531) (0)
Secondary Antibodies
| |
Isotype Controls
|
Additional Prolactin R Products
Research Areas for Prolactin R Antibody (NB300-531)
Find related products by research area.
|
Blogs on Prolactin R