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Prolactin R Antibody (T6) - Azide and BSA Free

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Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunohistochemistry-Paraffin: Prolactin R Antibody (T6) [NB300-531] - Normal biopsies of deparaffinized Rat pituitary gland tissue at a dilution of 1:50.
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunohistochemistry: Prolactin R Antibody (T6) [NB300-531] - Staining of PRL receptor in NB2 cells using NB300-531.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
T6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Prolactin R Antibody (T6) - Azide and BSA Free Summary

Immunogen
Purified rat liver PRL receptor.
Specificity
Detects prolactin (PRL) receptor in rat tissues. This does not cross-react with Growth Hormone (GH) receptor.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRLR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25 - 1:200
  • Immunohistochemistry-Paraffin 1:25 - 1:200
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 42 kDa protein representing PRL receptor in NB2 cell lysate.
Publications
Read Publications using NB300-531.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml PBS.

Alternate Names for Prolactin R Antibody (T6) - Azide and BSA Free

  • delta 4-delta 7/11 truncated prolactin receptor
  • delta 4-SF1b truncated prolactin receptor
  • hPRL receptor
  • hPRLrI
  • PRLR
  • PRL-R
  • Prolactin R
  • prolactin receptor delta 7/11
  • prolactin receptor
  • ProlactinR
  • secreted prolactin binding protein

Background

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin R Antibody (NB300-531)(7)


Showing Publications 1 - 7 of 7.
Publications using NB300-531 Applications Species
Aleman-Garcia YP, Vaquero-Garcia RM, Flores-Fernandez R et al. Prolactin Increases the Frequency of Follicular T Helper Cells with Enhanced IL21 Secretion and OX40 Expression in Lupus-Prone MRL/lpr Mice Journal of immunology research 2021-03-08 [PMID: 33763492]
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M. Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations. J Immunol. 1993 Jun 15;150(12):5673-81. 1993-01-01 [PMID: 8515082]
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endo., 134(5): 2108-2114. 1994-01-01 [PMID: 8156910]
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endo., 135(4): 1299-1306. 1994-01-01 [PMID: 7925093]
Okamura H, Zachwieja J, Raguet S, Kelly PA. Characterization and applications of monoclonal antibodies to the prolactin receptor. Endo., 124(5): 2499-2508. 1989-01-01 [PMID: 2707162]
Dardenne M, Kelly PA, Bach JF, Savino W. Identification and functional activity of prolactin receptors in thymic epithelial cells. Proc. Natl. Acad. Sci., USA, 88: 9700-9704. 1991-01-01 [PMID: 1946390]
Rozakis-Adcock M, Kelly PA. Mutational analysis of the ligand-binding domain of the prolactin receptor. J. Biol. Chem., 266(25): 16472-16477. 1991-01-01 [PMID: 1885580]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PRLR
Entrez
Uniprot