![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-18920231735520.jpg "Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Phospho-Profilin (Ser-138) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Ser-138 in human Profilin 1. This sequence is well conserved in rat and mouse Profilin 1. The serine site has some homology to the conserved site (Ser-138) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4. [UniProt# P07737] |
| Modification | p Ser138 |
| Specificity | This antibody was cross-adsorbed to unphosphorylated Profilin 1 (Ser-138) then affinity purified using Profilin 1 (Ser-138) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin 1 in human Jurkat and A431 cells, as well as rat PC12 cells. This reactivity is decreased by lambda phosphatase treatment, and is blocked by phospho-profilin (Ser-138) blocking peptide. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PFN1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Profilin 1 Antibody (NBP3-23342)Find related products by research area.
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Profiling the Profilin 1 Antibody Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod... Read full blog post. |
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| Gene Symbol | PFN1 |
![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKC alpha kinase then untreated (-) or treated with lambdaphosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-199202312523637.jpg "Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKC alpha kinase then untreated (-) or treated with lambdaphosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.")
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