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PRDM14 Antibody [Unconjugated]

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Western blot shows lysates of BG01V human embryonic stem cells (undifferentiated) and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM14 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of BG01V human embryonic stem cells (undifferentiated) and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human PRDM14 Antigen ...read more
PRDM14 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF6175-SP
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PRDM14 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PRDM14
Ala2-Asp201
Accession # Q9GZV8
Specificity
Detects human PRDM14 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PRDM14
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM14 Antibody [Unconjugated]

  • MGC59730
  • PFM11
  • PR domain containing 14
  • PR domain zinc finger protein 14
  • PR domain-containing protein 14
  • PRDM14
  • PR-domain zinc finger protein 14

Background

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400‑568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up‑regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRDM14
Uniprot