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Human PRAME - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human PRAME - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39439] - Standard Curve Reference
ELISA: Human PRAME - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39439] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.105 ng/mL (example only; lot dependent)

Order Details

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Human PRAME - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.105 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PRAME

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human PRAME - Ready-To-Use ELISA Kit (Colorimetric)

  • cancer/testis antigen 130
  • MAPEOIP4CT130
  • melanoma antigen preferentially expressed in tumors
  • OIP-4
  • Opa-interacting protein 4
  • Opa-interacting protein OIP4
  • preferentially expressed antigen in melanoma
  • Preferentially expressed antigen of melanoma

Background

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Manual - Human PRAME - Ready-To-Use ELISA Kit (Colorimetric)
  PDF Datasheet
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Bioinformatics

Gene Symbol PRAME