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Plexin A2 Antibody (583603)

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Western blot shows lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody ...read more
Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
583603
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5486-SP
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Plexin A2 Antibody (583603) Summary

Specificity
Detects mouse Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B2, D1, recombinant mouse Plexin A1, A3, B3, or C1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
PLXNA2
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using
MAB5486 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A2 Antibody (583603)

  • FLJ11751
  • FLJ30634
  • KIAA0463plexin 2
  • OCT
  • OCTSemaphorin receptor OCT
  • Plexin A2
  • PLXN2
  • PLXN2plexin-A2
  • PLXNA2
  • transmembrane protein OCT

Background

Plexin A2 (also OCT) is a 220-230 kDa member of the plexin-A subfamily, plexin family of proteins. It is found on cerebellar granule cells, sensory neurons, and cardiac neural crest cells. It participates in cell migration and axon guidance, and does so by serving as a receptor for Sema6A and B, and as a coreceptor with neuropilin-1 for Sema3A and C. Mature mouse Plexin A2 is an 1860 amino acid (aa) type I transmembrane glycoprotein. It contains a 1203 aa extracellular domain (ECD) and a 636 aa cytoplasmic region. The ECD contains one Sema domain (aa 35-508), three PSI domains (aa 510-855) and four IPT regions (aa 858-1228). There is one alternate start site 10 aa upstream of the standard start site. Over aa 35-1237, mouse Plexin A2 shares 96% aa sequence identity with human Plexin A2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Plexin A2 Antibody (MAB5486)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol PLXNA2
Uniprot