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Plectin Antibody (7A8)

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Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220] - Frozen sections acetone fixed Rat cardiac muscle tissue sections were stained with 1:200 Monoclonal Anti-Plectin antibody produced in Mouse, Clone: 7A8, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, MaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, mIF, MS
Clone
7A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Plectin Antibody (7A8) Summary

Immunogen
Plectin purified from rat glioma C6 cells.
Epitope
Monoclonal Anti-Plectin recognizes an epitope located in the middle section on the rod domain of the plectin molecule, approximately half way between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as thi
Specificity
The inhibits the interaction of plectin with vimentin and lamin B.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PLEC
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • Electron Microscopy
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
WB: Detects a band of approximately 300 kDa.
Publications
Read Publication using
NB120-11220 in the following applications:

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30591979).Cross-reacts with Marsupials. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Plectin Antibody (7A8)

  • EBS1
  • HD1
  • Hemidesmosomal protein 1
  • LGMD2Q
  • PCNplectin 1, intermediate filament binding protein, 500kD
  • PLEC1b
  • PLEC1EBSO
  • plectin 1, intermediate filament binding protein 500kDa
  • plectin
  • plectin-1
  • PLTNepidermolysis bullosa simplex 1 (Ogna)

Background

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Plectin Antibody (NB120-11220)(1)

We have publications tested in 1 confirmed species: Bovine.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Plectin Antibody (NB120-11220) (0)

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Bioinformatics

Gene Symbol PLEC
Entrez