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Pleckstrin Antibody (2D8)

Images

 
Western Blot: Pleckstrin Antibody (2D8) [H00005341-M03] - Analysis of PLEK expression in HL-60 (Cat # L014V1).
Immunocytochemistry/ Immunofluorescence: Pleckstrin Antibody (2D8) [H00005341-M03] - Analysis of monoclonal antibody to PLEK on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Pleckstrin Antibody (2D8) [H00005341-M03] - Detection limit for recombinant GST tagged PLEK is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2D8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Pleckstrin Antibody (2D8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PLEK (AAH18549, 121 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PETIDLGALYLSMKDTEKGIKELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENS
Specificity
PLEK - pleckstrin (2D8)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PLEK
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pleckstrin Antibody (2D8)

  • FLJ27168
  • p47
  • P47Platelet 47 kDa protein
  • pleckstrin

Background

Pleckstrin is the major protein kinase C substrate of platelets however its exact function is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pleckstrin Antibody (H00005341-M03) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLEK
Entrez
Uniprot