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PLC-gamma 1 Antibody (2F3D11) - BSA Free

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Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 39-181)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 39-181)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mAb against human PLCG1 (AA: 39-181) recombinant protein. (Expected MW is 43 kDa)
Western Blot: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis using PLCG1 mouse mAb against Jurkat (1), K562 (2), A431 (3), Hela (4), and PC-12 (5) cell lysate.
ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: PLC-gamma 1 Antibody (2F3D11) [NBP2-61790] - Analysis of Hela cells using PLCG1 mouse mAb (green) and negative control (red).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow
Clone
2F3D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-61790-0.025ml
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PLC-gamma 1 Antibody (2F3D11) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PLC-gamma 1 (AA: 39-181) expressed in E. Coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PLCG1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
Theoretical MW
89.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PLC-gamma 1 Antibody (2F3D11) - BSA Free

  • EC 3.1.4.11
  • gamma 1 (formerly subtype 148)
  • monophosphatidylinositol phosphodiesterase
  • NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
  • phosphatidylinositol phospholipase C
  • phosphoinositidase C
  • phosphoinositide phospholipase C
  • Phosphoinositide phospholipase C-gamma-1
  • phospholipase C, gamma 1
  • phospholipase C-148
  • Phospholipase C-gamma-1
  • Phospholipase C-II
  • PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
  • PLC148
  • PLC-148
  • PLCG1
  • PLCgamma 1
  • PLC-gamma 1
  • PLC-gamma-1
  • PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
  • PPLCA
  • triphosphoinositide phosphodiesterase

Background

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PLCG1