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PLC-beta 4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: PLC-beta 4 Antibody [NBP2-58912] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.
PLC-beta 4 Antibody [NBP2-58912] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
PLC-beta 4 Antibody [NBP2-58912]- Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
PLC-beta 4 Antibody [NBP2-58912] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
PLC-beta 4 Antibody [NBP2-58912] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PLC-beta 4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALASGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKL
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PLCB4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-P Retrieval method: HIER pH6.
Control Peptide
PLC-beta 4 Recombinant Protein Antigen (NBP2-58912PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PLC-beta 4 Antibody

  • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
  • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 4
  • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4
  • beta 4 (1-Phosphatidylinositol-4,5-BisphosphatePhosphodiesterase Beta 4))
  • EC 3.1.4.11
  • FLJ16169
  • inositoltrisphosphohydrolase
  • monophosphatidylinositol phosphodiesterase
  • phosphoinositidase C
  • phosphoinositide phospholipase C
  • phospholipase C, beta 4
  • PLCB4
  • PLCbeta 4
  • PLC-beta 4
  • triphosphoinositide phosphodiesterase

Background

The protein encoded by the Phospholipase C beta 4 gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PLCB4