![Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Western-Blot-NBP2-42686-img0003.jpg "Western Blot: PKM2 Antibody (1B10) [NBP2-42686] - Fig 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : MCF7 cell lysate Lane 4 : A549 cell lysate Lane 5 : 293T cell lysate Fig 2: The Recombinant human PKM2 (50ng) protein was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PKM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1B10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human PKM2 (1-531aa) purified from E.coli. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PKM |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
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![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0005.jpg "Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PKM2-Antibody-1B10-Immunocytochemistry-Immunofluorescence-NBP2-42686-img0004.jpg "Immunocytochemistry/Immunofluorescence: PKM2 Antibody (1B10) [NBP2-42686] - Analysis of PKM2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PKM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
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