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PIP Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining in human salivary gland and skeletal muscle tissues.. Corresponding PIP RNA-seq data are presented for the same tissues.
Western Blot: PIP Antibody [NBP1-83386] - Analysis in human cell line TD47D.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human salivary gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human skeletal muscle shows no positivity in myocytes.
Immunohistochemistry-Paraffin: PIP Antibody [NBP1-83386] - Staining of human duodenum shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Func, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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PIP Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FDIPKSVRPNDEVTAVLAVQTELKECMVVKTYLISSIPLQGAFNYKYTACLCDDNPKTFYWDFYTNRTVQIAAVVDVIRELGICPDDAAVIPIKNNRFYTIEILKVE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Proximity Ligation Assay Reported in scientific literature (PMID:34440877)
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PIP Protein (NBP1-83386PEP)
Publications
Read Publications using
NBP1-83386 in the following applications:

  • PLA
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PIP Antibody

  • GCDFP15prolactin-inducible protein
  • GCDFP-15SABP
  • GPIP4gp17
  • Gross cystic disease fluid protein 15
  • prolactin-induced proteinSecretory actin-binding protein

Background

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIP Antibody (NBP1-83386)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: PLA, WB.


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PLA
(1)
WB
(1)
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(2)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on PIP

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Bioinformatics

Gene Symbol PIP