![Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Western-Blot-NBP2-42526-img0002.jpg "Western Blot: Apolipoprotein D Antibody [NBP2-42526] - Analysis of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence EIEKIPTTFENGRC corresponding to internal region according to NP_001638.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOD |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in Flow reported in scientific literature (PMID:33334888). WB: Approx. 25 kDa band observed in human liver and kidney lysates (calculated MW of 21.3 kDa band according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form. Use in ICC/IF was reported in scientific literature (PMID: 31779197). |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Figure 4. ApoA1, ApoD (Apolipoprotein D), and LCAT immunolabeling in cultured keratocytes, dermal fibroblasts, and HepG2 liver cells. Cells were immunolabeled with ApoD (d-f) antibodies (green). DAPI was used to visualize DNA (blue). Anti-ApoD immunolabeled keratocytes (d) and dermal fibroblasts (e), but not HepG2 cells (f). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785/htm) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0005.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Figure 4. ApoA1, ApoD (Apolipoprotein D), and LCAT immunolabeling in cultured keratocytes, dermal fibroblasts, and HepG2 liver cells. Cells were immunolabeled with ApoD (d-f) antibodies (green). DAPI was used to visualize DNA (blue). Anti-ApoD immunolabeled keratocytes (d) and dermal fibroblasts (e), but not HepG2 cells (f). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785/htm) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunohistochemistry-Paraffin-NBP2-42526-img0003.jpg "Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Immunostaining of apolipoprotein (Apo)A1, ApoD (Apolipoprotein D), and lecithin:cholesterol acyltransferase (LCAT) in the human central cornea. Frozen sections from an individual cornea were incubated at 4 C with either anti-ApoA1 (a) or LCAT (o) antibodies (green). Nuclei were stained blue with DAPI. Antibodies were detected as described in the Materials and Methods. The epithelium, stromal keratocytes, and endothelium showed staining of LCAT (o). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42526-img0004.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein D Antibody [NBP2-42526] - Immunostaining of apolipoprotein (Apo)A1, ApoD (Apolipoprotein D), and lecithin:cholesterol acyltransferase (LCAT) in the human central cornea. Frozen sections from an individual cornea were incubated at 4 C with either anti-ApoA1 (a) or LCAT (o) antibodies (green). Nuclei were stained blue with DAPI. Antibodies were detected as described in the Materials and Methods. The epithelium, stromal keratocytes, and endothelium showed staining of LCAT (o). Image collected and cropped by CiteAb from the following publication (www.mdpi.com/2218-273X/9/12/785) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Apolipoprotein-D-Antibody-Immunohistochemistry-Paraffin-NBP2-42526-img0001.jpg "Immunohistochemistry-Paraffin: Apolipoprotein D Antibody [NBP2-42526] - Staining of paraffin embedded Human Kidney. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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